Linked Life Data

17979769

Source:http://linkedlifedata.com/resource/pubmed/id/17979769

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
16
pubmed:dateCreated
2007-11-5
pubmed:abstractText
Novel starting points for medicinal chemistry programmes can be effectively identified by screening libraries of fragment molecules in biochemical assays at high concentration. The key to success with this approach is the combination of a high quality fragment library with sensitive biochemical screening methods. There are an increasing number of literature reports where weakly active fragment molecules have been identified by high concentration biochemical assays. We have successfully demonstrated the use of high concentration screening of fragments, using a portfolio of single-molecule Fluorescence Correlation Spectroscopy (FCS+plus) detection techniques to ensure the highest reproducibility and sensitivity, and have determined the binding mode of active fragments to target proteins by X-ray crystallography. Further biophysical detection methods are reviewed for their applicability to studies of fragment binding.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1873-4294
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
7
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1582-91
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Fragment based drug discovery using fluorescence correlation: spectroscopy techniques: challenges and solutions.
pubmed:affiliation
Evotec, Schnackenburgallee 114, D-22525 Hamburg, Germany. Thomas.Hesterkamp@evotec.com
pubmed:publicationType
Journal Article, Review