Linked Life Data

17978832

Source:http://linkedlifedata.com/resource/pubmed/id/17978832

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2008-2-5
pubmed:abstractText
Adipose stem cells (ASCs) have the potential to differentiate into a variety of cell lineages both in vitro and in vivo. In this study, ASCs were harvested from normal Sprague-Dawley (SD) rats and transfected by BMP-2 gene before they were loaded on alginate. The ability of bone regeneration was determined in rat critical-size cranial defects. An 8-mm diameter defect was created in the calvarias of 36 rats; these rats were divided into three groups. In experimental group, the defects were filled with alginate gel combined with BMP-2 transfected ASCs; in negative control group, the defects were filled with alginate gel mixed with normal ASCs; in blank controls, the defects were filled with cell-free alginate gel. Four rats of each group were killed and the cranial defect sites were observed at 4, 8 and 16 weeks after surgery. There was complete repair of cranial defects in experimental group using the alginate gel loading BMP-2 transfected ASC, but only partial repair in negative controls and in the blank control. The engineering approach combining BMP-2 enhanced ASCs with alginate gel can therefore stimulate bone regeneration and repair for the large size bone defects.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0948-6143
pubmed:author
pubmed:issnType
Print
pubmed:volume
129
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
203-10
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Bone regeneration by BMP-2 enhanced adipose stem cells loading on alginate gel.
pubmed:affiliation
Department of Oral and Maxillofacial Surgery, West China College of Stomatology, Sichuan University, Chengdu 610041, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't