Source:http://linkedlifedata.com/resource/pubmed/id/17978210
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 11
|
| pubmed:dateCreated |
2007-11-5
|
| pubmed:databankReference | |
| pubmed:abstractText |
Novel halophilic, alkalithermophilic, Gram-type-positive bacterial strains were isolated from sediment of alkaline, hypersaline lakes of the Wadi An Natrun, Egypt. Cells of strain JW/NM-WN-LFT were rod-shaped, non-spore-forming and non-motile. Strain JW/NM-WN-LFT grew (at pH55 degrees C 9.5) between 35 and 56 degrees C, with an optimum at 53 degrees C. The pH55 degrees C range for growth was 8.3-10.6, with an optimum at pH55 degrees C 9.5 and no growth at pH55 degrees C 8.2 or below, or at pH55 degrees C 10.8 or above. At the optimum pH and temperature, the strain grew in the Na+ range of 3.1-4.9 M (1.5-3.3 M added NaCl) and optimally between 3.3 and 3.9 M Na+ (1.7-2.3 M added NaCl). Strain JW/NM-WN-LFT utilized fructose, cellobiose, ribose, trehalose, trimethylamine, pyruvate, Casamino acids, acetate, xylose and peptone as carbon and energy sources. Fumarate (20 mM), S2O3(2-) (20 mM), NO3- (20 mM) and iron(III) citrate (20 mM) were utilized as electron acceptors. During growth on sucrose, the isolate produced acetate and formate as major fermentation products. Main cellular fatty acids were iso-branched 15:0, i17:0 dimethylacetal and 16:0 dimethylacetal. The G+C content of genomic DNA was 40.4 mol% (HPLC). On the basis of genotypic and phenotypic characteristics, it is proposed that strain JW/NM-WN-LFT represents a novel genus and species, Natranaerobius thermophilus gen. nov., sp. nov. The type strain is JW/NM-WN-LFT (=DSM 18059T=ATCC BAA-1301T). Based on 16S rRNA gene sequence analysis, the strain forms a novel lineage within the class 'Clostridia' and clusters with uncultivated bacteria and unidentified strains retrieved from alkaline, hypersaline environments. The phylogenetic data suggest that the lineage represents a novel family, Natranaerobiaceae fam. nov., and order, Natranaerobiales ord. nov.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carbonates,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Ribosomal,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal, 16S,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/sodium carbonate
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
1466-5026
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
57
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2507-12
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:17978210-Bacterial Typing Techniques,
pubmed-meshheading:17978210-Carbonates,
pubmed-meshheading:17978210-DNA, Bacterial,
pubmed-meshheading:17978210-DNA, Ribosomal,
pubmed-meshheading:17978210-Egypt,
pubmed-meshheading:17978210-Fatty Acids,
pubmed-meshheading:17978210-Fresh Water,
pubmed-meshheading:17978210-Genes, rRNA,
pubmed-meshheading:17978210-Genotype,
pubmed-meshheading:17978210-Geologic Sediments,
pubmed-meshheading:17978210-Gram-Positive Bacteria,
pubmed-meshheading:17978210-Hot Temperature,
pubmed-meshheading:17978210-Hydrogen-Ion Concentration,
pubmed-meshheading:17978210-Molecular Sequence Data,
pubmed-meshheading:17978210-Phenotype,
pubmed-meshheading:17978210-Phylogeny,
pubmed-meshheading:17978210-RNA, Ribosomal, 16S,
pubmed-meshheading:17978210-Sequence Analysis, DNA,
pubmed-meshheading:17978210-Sodium Chloride
|
| pubmed:year |
2007
|
| pubmed:articleTitle |
Natranaerobius thermophilus gen. nov., sp. nov., a halophilic, alkalithermophilic bacterium from soda lakes of the Wadi An Natrun, Egypt, and proposal of Natranaerobiaceae fam. nov. and Natranaerobiales ord. nov.
|
| pubmed:affiliation |
Department of Microbiology, University of Georgia, Athens, GA 30602, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|