Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
45
pubmed:dateCreated
2007-11-7
pubmed:abstractText
Combining the experimental efficiency of a murine hepatic in vitro drug biotransformation system with in silico genetic analysis produces a model system that can rapidly analyze interindividual differences in drug metabolism. This model system was tested by using two clinically important drugs, testosterone and irinotecan, whose metabolism was previously well characterized. The metabolites produced after these drugs were incubated with hepatic in vitro biotransformation systems prepared from the 15 inbred mouse strains were measured. Strain-specific differences in the rate of 16 alpha-hydroxytestosterone generation and irinotecan glucuronidation correlated with the pattern of genetic variation within Cyp2b9 and Ugt1a loci, respectively. These computational predictions were experimentally confirmed using expressed recombinant enzymes. The genetic changes affecting irinotecan metabolism in mice mirrored those in humans that are known to affect the pharmacokinetics and incidence of adverse responses to this medication.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-10596902, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-1175644, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-12181437, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-12791308, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-15499019, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-16009447, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-16126366, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-16141793, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-16163395, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-16187025, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-16296948, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-16380716, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-16645459, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-16680137, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-16712705, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-16809730, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-16877259, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-17047491, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-2310412, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-3219345, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-3219346, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-7565971, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-8276413, http://linkedlifedata.com/resource/pubmed/commentcorrection/17978195-9621515
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
6
pubmed:volume
104
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17735-40
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
In silico and in vitro pharmacogenetic analysis in mice.
pubmed:affiliation
Department of Genetics and Genomics, Roche Palo Alto, Palo Alto, CA 94304, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural