Source:http://linkedlifedata.com/resource/pubmed/id/17977660
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2-3
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| pubmed:dateCreated |
2007-12-6
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| pubmed:abstractText |
The aim of this study was to clarify whether 12-lipoxygenase (12-LOX) activation was involved in reactive oxygen species (ROS) generation, extensive poly(ADP-ribose) polymerase (PARP) activation and neuronal death induced by glucose-deprivation, followed by glucose-reload (GD/R). The decrease of neuronal viability and accumulation of poly(ADP-ribose) induced by GD/R were prevented 3-aminobenzamide, a representative PARP inhibitor, demonstrating this treatment protocol caused the same oxidative stress with the previously reported one. The PARP activation, ROS generation and decrease of neuron viability induced by GD/R treatment were almost completely abolished by an extracellular zinc chelator, CaEDTA. p47(phox), a cytosolic component of NADPH oxidase was translocated the membrane fraction by GD/R, indicating its activation, but it did not generate detectable ROS. Surprisingly, pharmacological inhibition of NADPH oxidase with apocynin and AEBSF further decreased the decreased neuron viability induced by GD/R. On the other hand, AA861, a 12-LOX inhibitor, prevented ROS generation and decrease of neuron viability caused by GD/R. Interestingly, an antioxidant, N-acetyl-l-cysteine rescued the neurons from GD/R-induced oxidative stress, implying effectiveness of antioxidant administration. These findings suggested that activation of 12-LOX, but not NADPH oxidase, following to zinc release might play an important role in ROS generation and decrease of viability in GD/R-treated neurons.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adprt protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Antioxidants,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 12-Lipoxygenase,
http://linkedlifedata.com/resource/pubmed/chemical/Chelating Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/NADPH Oxidase,
http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Reactive Oxygen Species,
http://linkedlifedata.com/resource/pubmed/chemical/Zinc,
http://linkedlifedata.com/resource/pubmed/chemical/neutrophil cytosolic factor 1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
0304-3940
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
18
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| pubmed:volume |
429
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
120-5
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| pubmed:meshHeading |
pubmed-meshheading:17977660-Animals,
pubmed-meshheading:17977660-Antioxidants,
pubmed-meshheading:17977660-Arachidonate 12-Lipoxygenase,
pubmed-meshheading:17977660-Brain,
pubmed-meshheading:17977660-Brain Ischemia,
pubmed-meshheading:17977660-Cell Survival,
pubmed-meshheading:17977660-Cells, Cultured,
pubmed-meshheading:17977660-Chelating Agents,
pubmed-meshheading:17977660-Enzyme Activation,
pubmed-meshheading:17977660-Enzyme Inhibitors,
pubmed-meshheading:17977660-Glucose,
pubmed-meshheading:17977660-Hypoglycemia,
pubmed-meshheading:17977660-NADPH Oxidase,
pubmed-meshheading:17977660-Neurons,
pubmed-meshheading:17977660-Oxidative Stress,
pubmed-meshheading:17977660-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:17977660-Rats,
pubmed-meshheading:17977660-Reactive Oxygen Species,
pubmed-meshheading:17977660-Zinc
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| pubmed:year |
2007
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| pubmed:articleTitle |
Possible involvement of 12-lipoxygenase activation in glucose-deprivation/reload-treated neurons.
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| pubmed:affiliation |
Department of Environmental Biochemistry, Kyoto Pharmaceutical University, Misasagi, Kyoto 607-8414, Japan. nagasawa@mb.kyoto-phu.ac.jp
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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