Linked Life Data

17973238

Source:http://linkedlifedata.com/resource/pubmed/id/17973238

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-11-13
pubmed:abstractText
The purpose of this study was to define a biomarker panel for detection of cancer cells in cytologically negative sputum and to evaluate the panel for assessment of lung cancer risk. We examined 19 genetic and epigenetic markers using a sensitive fluorescence-based method in cytologically negative sputum and in lung tumour tissues from 82 lung cancer patients. We also used these markers to test the sputum of 37 cancer-free individuals who were matched by age, sex, and smoking habit. Based on the concordance of biomarkers in lung tumours and corresponding sputum, and the low prevalence in cancer-free individuals, we selected seven markers for a nested case-control study: microsatellite instability of D9S942; loss of heterozygosity of D9S286, D9S942, GATA49D12, and D13S170; and methylation of p16INK4a and RARbeta. Based on the assumption that a lung cancer cell has alterations in two or more of the seven biomarkers, we compared the pattern of biomarker alteration in lung tumours and corresponding sputum. Our comparison yielded a sensitivity of 82%, specificity of 75%, and concordance of 79%. Three cancer-free individuals were considered to have an elevated risk based on the criterion that their sputum showed alteration in two of the seven biomarkers. One individual was indeed diagnosed as having lung cancer 18 months after sputum collection. In the nested case-control study, six biomarkers showed significantly increased odds ratios ranging from 3.14 to 11.24. Our study defines a biomarker panel for detection of cancer cells in cytologically negative sputum and verifies its use for risk assessment of lung cancer. In combination with conventional diagnostic tools, this multiple genetic and epigenetic panel should improve the detection or risk assessment of lung cancer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0022-3417
pubmed:author
pubmed:copyrightInfo
(c) 2007 Pathological Society of Great Britain and Ireland
pubmed:issnType
Print
pubmed:volume
213
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
412-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17973238-Acid Anhydride Hydrolases, pubmed-meshheading:17973238-Base Sequence, pubmed-meshheading:17973238-Carcinoma, Non-Small-Cell Lung, pubmed-meshheading:17973238-Case-Control Studies, pubmed-meshheading:17973238-DNA Methylation, pubmed-meshheading:17973238-Epigenesis, Genetic, pubmed-meshheading:17973238-Female, pubmed-meshheading:17973238-Genes, p16, pubmed-meshheading:17973238-Genetic Markers, pubmed-meshheading:17973238-Humans, pubmed-meshheading:17973238-Loss of Heterozygosity, pubmed-meshheading:17973238-Lung Neoplasms, pubmed-meshheading:17973238-Male, pubmed-meshheading:17973238-Microsatellite Instability, pubmed-meshheading:17973238-Molecular Sequence Data, pubmed-meshheading:17973238-Neoplasm Proteins, pubmed-meshheading:17973238-Neoplasm Staging, pubmed-meshheading:17973238-Promoter Regions, Genetic, pubmed-meshheading:17973238-Receptors, Retinoic Acid, pubmed-meshheading:17973238-Risk Assessment, pubmed-meshheading:17973238-Sensitivity and Specificity, pubmed-meshheading:17973238-Sputum, pubmed-meshheading:17973238-Tumor Markers, Biological
pubmed:year
2007
pubmed:articleTitle
Multiple genetic and epigenetic biomarkers for lung cancer detection in cytologically negative sputum and a nested case-control study for risk assessment.
pubmed:affiliation
Division of Thoracic Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies