Linked Life Data

17970117

Source:http://linkedlifedata.com/resource/pubmed/id/17970117

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-10-31
pubmed:abstractText
A high performance liquid chromatographic (HPLC) method with gradient elution was developed for the simultaneous determination of vindoline, catharanthine and anhydrovinblastine in Catharanthus roseus. The analytes were separated on a Waters (5)C18-MS-II column (4.6 mm x 250 mm) and detected at 220 nm. The mobile phases were methanol-1% (v/v) diethylamine solution (adjusted to pH 7.3 with phosphate) for gradient elution at temperature of 25 degrees C. The calibration curves for vindoline, catharanthine and anhydrovinblastine showed good linearity in the ranges of 0.03-1 mg/mL (r = 0.9997), 0.03-1 mg/mL (r = 0.9999) and 0.01-0.5 mg/mL (r = 0.9986), respectively. The recoveries were 96.8%, 97.0%, and 96.4% and the relative standard deviations (RSDs) were 1.53%, 1.37%, and 1.96%. The method is accurate, rapid and simple for the determination of vindoline, catharanthine and anhydrovinblastine in Catharanthus roseus.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
1000-8713
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
550-2
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
[Simultaneous determination of vindoline, catharanthine and anhydrovinblastine in Catharanthus roseus by high performance liquid chromatography].
pubmed:affiliation
Key Laboratory of Forest Plant Ecology of Ministry of Education, Northeast Forestry University, Harbin 150040, China. alkaloid2004@126.com
pubmed:publicationType
Journal Article, English Abstract