Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2007-12-17
pubmed:abstractText
To investigate the mechanism of radioresistance of solid tumor cells, we created two expression vectors encoding Survivin mutants, T34A and D53A. When T34A and D53A were overexpressed in NIH3T3, A549 and HeLa cells, radiation-induced apoptosis was significantly enhanced. Furthermore, we examined the binding capability of Survivin with Smac/DIABLO in the cells that overexpressed these mutants. Coimmunoprecipitation analysis revealed that mutant form of Survivin, D53A and T34A could bind to Smac/DIABLO, but with much less affinity compared to the authentic form. These results suggest that radiation-induced apoptosis of tumor cells is increased by inhibition of the interaction between Survivin and Smac/DIABLO through overexpression of T34A and D53A.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0304-3835
pubmed:author
pubmed:issnType
Print
pubmed:day
18
pubmed:volume
259
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
71-81
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:17967504-Adenoviridae, pubmed-meshheading:17967504-Animals, pubmed-meshheading:17967504-Apoptosis, pubmed-meshheading:17967504-Caspases, pubmed-meshheading:17967504-Cytochromes c, pubmed-meshheading:17967504-Genetic Vectors, pubmed-meshheading:17967504-HeLa Cells, pubmed-meshheading:17967504-Humans, pubmed-meshheading:17967504-Inhibitor of Apoptosis Proteins, pubmed-meshheading:17967504-Intracellular Signaling Peptides and Proteins, pubmed-meshheading:17967504-Mice, pubmed-meshheading:17967504-Microtubule-Associated Proteins, pubmed-meshheading:17967504-Mitochondria, pubmed-meshheading:17967504-Mitochondrial Proteins, pubmed-meshheading:17967504-Mutation, pubmed-meshheading:17967504-NIH 3T3 Cells, pubmed-meshheading:17967504-Neoplasm Proteins, pubmed-meshheading:17967504-Neoplasms, pubmed-meshheading:17967504-Phosphorylation, pubmed-meshheading:17967504-Radiation Tolerance, pubmed-meshheading:17967504-Signal Transduction, pubmed-meshheading:17967504-Time Factors, pubmed-meshheading:17967504-Transfection, pubmed-meshheading:17967504-X-Rays
pubmed:year
2008
pubmed:articleTitle
Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO.
pubmed:affiliation
Laboratory of Radiation Biology, Department of Environmental Veterinary Medical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Sapporo 060-0818, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't