Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
44
pubmed:dateCreated
2007-11-5
pubmed:abstractText
Among the multiple steps constituting the kinesin mechanochemical cycle, one of the most interesting events is observed when kinesins move an 8-nm step from one microtubule (MT)-binding site to another. The stepping motion that occurs within a relatively short time scale ( approximately 100 mus) is, however, beyond the resolution of current experiments. Therefore, a basic understanding to the real-time dynamics within the 8-nm step is still lacking. For instance, the rate of power stroke (or conformational change) that leads to the undocked-to-docked transition of neck-linker is not known, and the existence of a substep during the 8-nm step still remains a controversial issue in the kinesin community. By using explicit structures of the kinesin dimer and the MT consisting of 13 protofilaments, we study the stepping dynamics with varying rates of power stroke (k(p)). We estimate that k(p)(-1) less, similar 20 micros to avoid a substep in an averaged time trace. For a slow power stroke with k(p)(-1) > 20 micros, the averaged time trace shows a substep that implies the existence of a transient intermediate, which is reminiscent of a recent single-molecule experiment at high resolution. We identify the intermediate as a conformation in which the tethered head is trapped in the sideway binding site of the neighboring protofilament. We also find a partial unfolding (cracking) of the binding motifs occurring at the transition state ensemble along the pathways before binding between the kinesin and MT.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-10408448, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-10557288, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-10617199, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-10908673, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-11283618, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-11427717, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-12591957, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-14566052, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-14634664, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-14657506, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-14684828, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-15102452, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-15102453, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-15276184, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-15738986, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-15794634, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-15902249, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-16230609, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-16408074, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-16698919, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-16698928, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-16783374, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-17013387, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-17135353, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-17163836, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-17243791, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-17287347, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-17325011, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-2412134, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-3926325, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-7548088, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-8099076, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-8110800, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-8369439, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-8580344, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-8700858, http://linkedlifedata.com/resource/pubmed/commentcorrection/17959770-9454569
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
30
pubmed:volume
104
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
17382-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Mechanical control of the directional stepping dynamics of the kinesin motor.
pubmed:affiliation
Department of Physics, University of California at San Diego, La Jolla, CA 92093-0374, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.