Linked Life Data

17956668

Source:http://linkedlifedata.com/resource/pubmed/id/17956668

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-10-24
pubmed:abstractText
This study was purposed to investigate whether the mdr1 participates in antiapoptotic mechanisms of hematologic malignant cells regulated by NF-kappaB and to explore the reversal effect of inhibiting NF-kappaB on drug-resistance of hematologic malignant cells so as to search a novel way for treatment of refractory leukemia. NF-kappaB activity and P-gp expression in K562/A02 cells cultured with PDTC in different concentration or different time were detected by immunohistochemistry and computerized picture analysis. The mdr1 was detected by reverse transcription-polymerase chain reaction, and the relationship among NF-kappaB, P-gp and mdr1 was analyzed. The results showed that in K562/A02 cells the expression of P-gp and mdr1 were positively correlated with NF-kappaB/p65, which showed the concentration and time dependent manner. It is concluded the mechanism of NF-kappaB regulating anti-apoptosis in K562/A02 cells has the correlation with the expression of mdr1 and P-gp. The expression of mdr 1 mRNA and P-gp can be reduced through inhibiting NF-kappaB in K562/A02 cells, so that the sensitivity of chemical therapy can be enhanced on hematologic malignant cells.
pubmed:language
chi
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1009-2137
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
950-4
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
[NF-kappaB regulating expression of mdr1 gene and P-gp to reverse drug-resistance in leukemic cells].
pubmed:affiliation
Department of Hematology, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an 710061, China.
pubmed:publicationType
Journal Article, English Abstract