Source:http://linkedlifedata.com/resource/pubmed/id/17955388
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2007-11-9
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| pubmed:abstractText |
Androgen and the androgen receptor (AR) have been shown to play critical roles in male fertility. Our previous data demonstrated that mice lacking AR (AR(-/y)) revealed incomplete germ cell development and lowered serum testosterone levels, which resulted in azoospermia and infertility. However, the consequences of AR loss in Leydig cells remain largely unknown. Using a Cre-LoxP conditional knockout strategy, we generated a tissue-specific knockout mouse (L-AR(-/y)) with the AR gene deleted by the anti-Müllerian hormone receptor-2 (Amhr2) promoter driven Cre expressed in Leydig cells. Phenotype analyses show that the outside appearance of L-AR(-/y) mice was indistinguishable from wild type mice (AR(+/y)), but with atrophied testes and epididymis. L-AR(-/y) mice were infertile, with spermatogenic arrest predominately at the round spermatid stage and no sperm could be detected in the epididymis. L-AR(-/y) mice also have lower serum testosterone concentrations and higher serum leuteinizing hormone and follicle-stimulating hormone concentrations than AR(+/y) mice. Further mechanistic studies demonstrated that hypotestosteronemia in L-AR(-/y) mice is not caused by reducing numbers of Leydig cells, but instead by the alterations of several key steroidogenic enzymes, including 17beta-HSD3, 3beta-HSD6, and P450c17. Together, L-AR(-/y) mice provide in vivo evidence that functional AR in Leydig cells is essential to maintain normal spermatogenesis, testosterone production, and required for normal male fertility.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/17-Hydroxysteroid Dehydrogenases,
http://linkedlifedata.com/resource/pubmed/chemical/3 (or 17)-beta-hydroxysteroid...,
http://linkedlifedata.com/resource/pubmed/chemical/3-Hydroxysteroid Dehydrogenases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Androgen,
http://linkedlifedata.com/resource/pubmed/chemical/Steroid 17-alpha-Hydroxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Testosterone
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| pubmed:status |
MEDLINE
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| pubmed:month |
Aug
|
| pubmed:issn |
1355-008X
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| pubmed:author |
pubmed-author:AltuwaijriSalehS,
pubmed-author:ChangChawnshangC,
pubmed-author:ChenLu-MinLM,
pubmed-author:ChenYen-TaYT,
pubmed-author:ChiangHan-SunHS,
pubmed-author:ChuangKuang-HsiangKH,
pubmed-author:LinHung-YunHY,
pubmed-author:WangRuey-ShenRS,
pubmed-author:XuQingquanQ,
pubmed-author:YehShauh-DerSD,
pubmed-author:YehShuyuanS,
pubmed-author:YuI-ChenIC,
pubmed-author:ZhangCaixiaC
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| pubmed:issnType |
Print
|
| pubmed:volume |
32
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
96-106
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| pubmed:dateRevised |
2010-6-24
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| pubmed:meshHeading |
pubmed-meshheading:17955388-17-Hydroxysteroid Dehydrogenases,
pubmed-meshheading:17955388-3-Hydroxysteroid Dehydrogenases,
pubmed-meshheading:17955388-Animals,
pubmed-meshheading:17955388-Apoptosis,
pubmed-meshheading:17955388-Azoospermia,
pubmed-meshheading:17955388-Cell Proliferation,
pubmed-meshheading:17955388-Epididymis,
pubmed-meshheading:17955388-Female,
pubmed-meshheading:17955388-Gene Expression,
pubmed-meshheading:17955388-Leydig Cells,
pubmed-meshheading:17955388-Male,
pubmed-meshheading:17955388-Mice,
pubmed-meshheading:17955388-Mice, Inbred C57BL,
pubmed-meshheading:17955388-Mice, Knockout,
pubmed-meshheading:17955388-Organ Size,
pubmed-meshheading:17955388-Receptors, Androgen,
pubmed-meshheading:17955388-Spermatids,
pubmed-meshheading:17955388-Spermatogenesis,
pubmed-meshheading:17955388-Steroid 17-alpha-Hydroxylase,
pubmed-meshheading:17955388-Testis,
pubmed-meshheading:17955388-Testosterone
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| pubmed:year |
2007
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| pubmed:articleTitle |
Infertility with defective spermatogenesis and steroidogenesis in male mice lacking androgen receptor in Leydig cells.
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| pubmed:affiliation |
Department of Pathology, the Cancer Center, University of Rochester, 601 Elmwood Ave, Box 626, Rochester, NY 14642, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
|