Source:http://linkedlifedata.com/resource/pubmed/id/17955272
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2008-1-24
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| pubmed:abstractText |
Alternative splicing is an important mechanism to generate a large number of mRNAs, thus increasing proteome diversity and tissue specificity. Three transcript variants of alpha-synuclein, a neuronal protein mainly involved in synapses, have been described so far. Whereas alpha-synuclein 140 is the whole and main transcript, alpha-synuclein 112 and 126 are short proteins that result from in-frame deletions of exons 3 and 5, respectively. Because the aforesaid alpha-synuclein isoforms show differential expression changes in Lewy body diseases (LBDs), in the present work, we searched for a fourth alpha-synuclein isoform and studied its expression levels in LBD brains. By using isoform-specific primers, isoform co-amplification and direct sequencing, we identified alpha-synuclein 98, which lacks exons 3 and 5. mRNA expression analyses in non-neuronal tissue revealed that alpha-synuclein 98 is a brain-specific splice variant with varying expression levels in different areas of fetal and adult brain. Additionally, we studied alpha-synuclein 98 expression levels by real-time semi-quantitative RT-PCR in the frontal cortices of LBD patients and compared them with those of Alzheimer disease (AD) patients and control subjects. Overexpression of alpha-synuclein 98 in LBD and AD brains would indicate its specific involvement in the pathogenesis of these neurodegenerative disorders.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/SNCA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Synuclein
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| pubmed:status |
MEDLINE
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| pubmed:month |
Feb
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| pubmed:issn |
1364-6753
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
9
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
15-23
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| pubmed:meshHeading |
pubmed-meshheading:17955272-Adult,
pubmed-meshheading:17955272-Alternative Splicing,
pubmed-meshheading:17955272-Alzheimer Disease,
pubmed-meshheading:17955272-Amino Acid Sequence,
pubmed-meshheading:17955272-Base Sequence,
pubmed-meshheading:17955272-Brain,
pubmed-meshheading:17955272-Case-Control Studies,
pubmed-meshheading:17955272-DNA Primers,
pubmed-meshheading:17955272-Exons,
pubmed-meshheading:17955272-Fetus,
pubmed-meshheading:17955272-Frontal Lobe,
pubmed-meshheading:17955272-Gene Expression,
pubmed-meshheading:17955272-Humans,
pubmed-meshheading:17955272-Lewy Body Disease,
pubmed-meshheading:17955272-Molecular Sequence Data,
pubmed-meshheading:17955272-Protein Isoforms,
pubmed-meshheading:17955272-RNA, Messenger,
pubmed-meshheading:17955272-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:17955272-Sequence Homology, Amino Acid,
pubmed-meshheading:17955272-alpha-Synuclein
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| pubmed:year |
2008
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| pubmed:articleTitle |
Identification and characterization of a new alpha-synuclein isoform and its role in Lewy body diseases.
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| pubmed:affiliation |
Department of Pathology, Hospital Universitari Germans Trias i Pujol, Autonomous University of Barcelona, Barcelona, Spain. katrinbeyer@hotmail.com
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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