Linked Life Data

17954234

Source:http://linkedlifedata.com/resource/pubmed/id/17954234

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2007-10-23
pubmed:abstractText
The corona discharge used to generate positive and negative ions under conventional atmospheric pressure chemical ionization (APCI) conditions also provides a source of low-energy gas-phase electrons. This is thought to occur by displacement of electrons from the nitrogen sheath gas. Therefore, suitable analytes can undergo electron capture in the gas phase in a manner similar to that observed for gas chromatography/electron capture negative chemical ionization/mass spectrometry (MS). This technique, which has been named electron-capture APCI (ECAPCI)/MS, mass spectrometry provides an increase in sensitivity of two orders of magnitude when compared with conventional APCI methodology. It is a simple procedure to tag arachidonic acid- and linoleic acid-derived oxidized lipids with an electron-capturing group such as the pentafluorobenzyl (PFB) moiety before analysis. PFB derivatives have previously been used as electron-capturing derivatives because they undergo dissociative electron capture in the gas phase to generate negative ions through the loss of a PFB radical. A similar process occurs under ECAPCI conditions. By monitoring the negative ions that are formed, it is possible to obtain extremely high sensitivity for PFB derivatives of oxidized lipids derived from arachidonic and linoleic acid. A combination of stable isotope dilution methodology and chiral liquid chromatography-ECAPCI/MS makes it possible to resolve and quantify complex mixtures of regioisomeric and enantiomeric oxidized lipids.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0076-6879
pubmed:author
pubmed:issnType
Print
pubmed:volume
433
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
159-74
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Targeted chiral lipidomics analysis by liquid chromatography electron capture atmospheric pressure chemical ionization mass spectrometry (LC-ECAPCI/MS).
pubmed:affiliation
Centers for Cancer Pharmacology and Excellence in Environmental Toxicology, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural