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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2008-1-15
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pubmed:abstractText |
The viral strain responsible for smallpox infection is variola major (VARV). As a result of the successful eradication of smallpox with the vaccinia virus (VACV), the general population is no longer required to receive a smallpox vaccine, and will have no protection against smallpox. This lack of immunity is a concern due to the potential for use of smallpox as a biological weapon. Considerable progress has been made in the development of subunit-based smallpox vaccines resulting from the identification of VACV protective antigens. It also offers the possibility of using antigens from VARV to formulate the next generation subunit-based smallpox vaccines. Here, we show that codon-optimized DNA vaccines expressing three VARV antigens (A30, B7 and F8) and their recombinant protein counterparts elicited high-titer, cross-reactive, VACV neutralizing antibody responses in mice. Vaccinated mice were protected from intraperitoneal and intranasal challenges with VACV. These results suggest the feasibility of a subunit smallpox vaccine based on VARV antigen sequences to induce immunity against poxvirus infection.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-10573171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-10639319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-11668342,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-11923489,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-12124461,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-12620810,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-12869693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-15070762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-15078924,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-15308359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-15308360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-15346340,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-15367588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-15491873,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-15650214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-15837236,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-15951823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-16123129,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-16140431,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-16140750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-16256161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-16333009,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-16888017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-16919703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-17098336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-17240007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-7483290,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-7625123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-8195718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-8805248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17950773-9927575
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Smallpox Vaccine,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Attenuated,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Vaccines
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0042-6822
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
371
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
98-107
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pubmed:dateRevised |
2011-5-4
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pubmed:meshHeading |
pubmed-meshheading:17950773-Amino Acid Sequence,
pubmed-meshheading:17950773-Animals,
pubmed-meshheading:17950773-Antigens, Viral,
pubmed-meshheading:17950773-Body Weight,
pubmed-meshheading:17950773-Cell Line,
pubmed-meshheading:17950773-Chemoprevention,
pubmed-meshheading:17950773-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:17950773-Escherichia coli,
pubmed-meshheading:17950773-Feasibility Studies,
pubmed-meshheading:17950773-Female,
pubmed-meshheading:17950773-Humans,
pubmed-meshheading:17950773-Immunization Schedule,
pubmed-meshheading:17950773-Immunoglobulin G,
pubmed-meshheading:17950773-Kidney,
pubmed-meshheading:17950773-Mice,
pubmed-meshheading:17950773-Mice, Inbred BALB C,
pubmed-meshheading:17950773-Models, Animal,
pubmed-meshheading:17950773-Molecular Sequence Data,
pubmed-meshheading:17950773-Neutralization Tests,
pubmed-meshheading:17950773-Sequence Homology, Amino Acid,
pubmed-meshheading:17950773-Smallpox,
pubmed-meshheading:17950773-Smallpox Vaccine,
pubmed-meshheading:17950773-Vaccines, Attenuated,
pubmed-meshheading:17950773-Vaccines, DNA,
pubmed-meshheading:17950773-Vaccines, Subunit,
pubmed-meshheading:17950773-Vaccinia virus,
pubmed-meshheading:17950773-Variola virus,
pubmed-meshheading:17950773-Viral Vaccines
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pubmed:year |
2008
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pubmed:articleTitle |
Immunogenicity and protection efficacy of subunit-based smallpox vaccines using variola major antigens.
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pubmed:affiliation |
Laboratory of Nucleic Acid Vaccines, Department of Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA.
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