Source:http://linkedlifedata.com/resource/pubmed/id/17944303
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2007-10-19
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pubmed:abstractText |
A study aimed at validating a human progesterone enzyme immunoassay kit was carried out on cattle at Bambui, Cameroon. Progesterone ELISA Kits (EH-511) were obtained from Clinpro International. Forty-one cows were selected, of which 19 were pregnant and 22 within 14 days post partum. Blood samples were analysed and progesterone levels were deduced from a curve obtained from standard absorbance values (A450). Results show that 95.5% of postpartum cows had progesterone levels below 1 ng/ ml, with the highest level being 0.75 ng/ml. The mean level was 0.5 +/- 0.26 ng/ml. The cows in the 'pregnant group' had progesterone levels ranging from 3.5 to 17.5 ng/ml. This kit can be used for measuring progesterone levels in cattle. Levels of 1 ng/ml for two consecutive samples or one sample at or above 3 ng/ml are an indication of the presence of corpus luteum, while cows below 1 ng/ml will be in anoestrus.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0049-4747
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
335-8
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pubmed:meshHeading |
pubmed-meshheading:17944303-Animals,
pubmed-meshheading:17944303-Cameroon,
pubmed-meshheading:17944303-Cattle,
pubmed-meshheading:17944303-Female,
pubmed-meshheading:17944303-Immunoenzyme Techniques,
pubmed-meshheading:17944303-Pregnancy,
pubmed-meshheading:17944303-Pregnancy Tests,
pubmed-meshheading:17944303-Progesterone,
pubmed-meshheading:17944303-Reproducibility of Results
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pubmed:year |
2007
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pubmed:articleTitle |
Validation of a human progesterone enzyme immunoassay (EIA) kit for use on serum of cattle in Cameroon.
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pubmed:affiliation |
Institute of Agricultural Research for Development, Bambui B.P. 51, Bamenda, Cameroon. hbayemi@yahoo.fr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Validation Studies
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