Linked Life Data

17942926

Source:http://linkedlifedata.com/resource/pubmed/id/17942926

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
20
pubmed:dateCreated
2007-10-18
pubmed:abstractText
Cancer chemopreventive agent sulforaphane (SFN) and dibenzoylmethane (DBM) showed antitumorigenesis effects in several rodent carcinogenesis models. In this study, we investigated the cancer chemopreventive effects and the underlying molecular mechanisms of dietary administration of SFN and DBM alone or in combination in the ApcMin/+ mice model. Male ApcMin/+ mice (12 per group) at age of 5 weeks were given control AIN-76A diet, diets containing 600 ppm SFN and 1.0% DBM, or a combination of 300 ppm SFN and 0.5% DBM for 10 weeks. Mice were then sacrificed, and tumor numbers and size were examined. Microarray analysis, Western blotting, ELISA, and immunohistochemical staining were done to investigate the underlying molecular mechanisms of cancer chemopreventive effects of SFN and DBM. Dietary administrations of SFN and DBM alone or in combination significantly inhibited the development of intestinal adenomas by 48% (P=0.002), 50% (P=0.001), and 57% (P<0.001), respectively. Dietary administration of 600 ppm SFN and 1.0% DBM also reduced colon tumor numbers by 80% (P=0.016) and 60% (P=0.103), respectively, whereas the combination of SFN and DBM treatment blocked the colon tumor development (P=0.002). Both SFN and DBM treatments resulted in decreased levels of prostaglandin E2 or leukotriene B4 in intestinal polyps or apparently normal mucosa. Treatments also led to the inhibition of cell survival and growth-related signaling pathways (such as Akt and extracellular signal-regulated kinase) or biomarkers (such as cyclooxygenase-2, proliferating cell nuclear antigen, cleaved caspases, cyclin D1, and p21). In conclusion, our results showed that both SFN and DBM alone as well as their combination are potent natural dietary compounds for chemoprevention of gastrointestinal cancers.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
67
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9937-44
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:17942926-Adenomatous Polyposis Coli, pubmed-meshheading:17942926-Animals, pubmed-meshheading:17942926-Anticarcinogenic Agents, pubmed-meshheading:17942926-Antineoplastic Combined Chemotherapy Protocols, pubmed-meshheading:17942926-Apoptosis, pubmed-meshheading:17942926-Arachidonic Acid, pubmed-meshheading:17942926-Body Weight, pubmed-meshheading:17942926-Cell Cycle Proteins, pubmed-meshheading:17942926-Chalcones, pubmed-meshheading:17942926-Diet, pubmed-meshheading:17942926-Gene Expression Profiling, pubmed-meshheading:17942926-Intestine, Small, pubmed-meshheading:17942926-Male, pubmed-meshheading:17942926-Mice, pubmed-meshheading:17942926-Mitogen-Activated Protein Kinases, pubmed-meshheading:17942926-Phosphorylation, pubmed-meshheading:17942926-Polymerase Chain Reaction, pubmed-meshheading:17942926-Proto-Oncogene Proteins c-akt, pubmed-meshheading:17942926-Thiocyanates
pubmed:year
2007
pubmed:articleTitle
Chemoprevention of familial adenomatous polyposis by natural dietary compounds sulforaphane and dibenzoylmethane alone and in combination in ApcMin/+ mouse.
pubmed:affiliation
Center for Cancer Prevention Research, Department of Pharmaceutics, and Susan Lehman Cullman Laboratory for Cancer Research, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey, Piscataway, New Jersey 08854, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural