Linked Life Data

17940963

Source:http://linkedlifedata.com/resource/pubmed/id/17940963

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2007-10-17
pubmed:abstractText
Ebola virus VP40 contains 2 overlapping late domains (7-PTAP-10 and 10-PPEY-13) that are essential for its interaction with Tsg101 and Nedd4 in the promotion of viral egress. Deletion of the late domains inhibits VP40-induced virus-like particles (VLPs). However, a truncated form of VP40, which lacks a late domain because of the deletion of amino acids 1-30, is released into supernatant as a VLP, indicating that the remaining portion of VP40 contains the structural elements required for VLP release. Thus, the purpose of this study was to identify the VP40 sequence essential for VLP budding, through the generation of deletion and alanine-scanning mutants. We found that the amino acid sequence around the proline at position 53 plays a critical role in VLP production and intracellular transport. These data also may suggest that a novel host factor(s) is involved in virus budding.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1899
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
196 Suppl 2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
S291-5
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Mapping of a region of Ebola virus VP40 that is important in the production of virus-like particles.
pubmed:affiliation
Division of Virology, Department of Microbiology and Immunology, University of Tokyo, Tokyo 108-8639, Japan.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural