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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
2007-10-16
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pubmed:abstractText |
Two forms of RNA Polymerase IV (PolIVa/PolIVb) have been implicated in RNA-directed DNA methylation (RdDM) in Arabidopsis. Prevailing models imply a distinct function for PolIVb by association of Argonaute4 (AGO4) with the C-terminal domain (CTD) of its largest subunit NRPD1b. Here we show that the extended CTD of NRPD1b-type proteins exhibits conserved Argonaute-binding capacity through a WG/GW-rich region that functionally distinguishes Pol IVb from Pol IVa, and that is essential for RdDM. Site-specific mutagenesis and domain-swapping experiments between AtNRPD1b and the human protein GW182 demonstrated that reiterated WG/GW motifs form evolutionarily and functionally conserved Argonaute-binding platforms in RNA interference (RNAi)-related components.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-11278562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-11950943,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-12202748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-12522258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-12791996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-15372043,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-15766525,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-15924141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-16109369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-16140984,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-16169483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-16284622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-16284623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-16567016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-16815998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-16839878,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-16839879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-16880270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-17183357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-17320512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-17360559,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-17531816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-17560368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-3670292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-8566758,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-8991083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-9254694,
http://linkedlifedata.com/resource/pubmed/commentcorrection/17938239-9811807
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0890-9369
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
21
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2539-44
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:17938239-Amino Acid Motifs,
pubmed-meshheading:17938239-Amino Acid Sequence,
pubmed-meshheading:17938239-Arabidopsis,
pubmed-meshheading:17938239-Arabidopsis Proteins,
pubmed-meshheading:17938239-Argonaute Proteins,
pubmed-meshheading:17938239-Conserved Sequence,
pubmed-meshheading:17938239-DNA Methylation,
pubmed-meshheading:17938239-DNA-Directed RNA Polymerases,
pubmed-meshheading:17938239-Evolution, Molecular,
pubmed-meshheading:17938239-Humans,
pubmed-meshheading:17938239-Molecular Sequence Data,
pubmed-meshheading:17938239-Mutagenesis, Site-Directed,
pubmed-meshheading:17938239-Plants, Genetically Modified,
pubmed-meshheading:17938239-Protein Binding,
pubmed-meshheading:17938239-Protein Structure, Tertiary,
pubmed-meshheading:17938239-Protein Subunits,
pubmed-meshheading:17938239-RNA Interference,
pubmed-meshheading:17938239-Repetitive Sequences, Amino Acid,
pubmed-meshheading:17938239-Sequence Homology, Amino Acid
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pubmed:year |
2007
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pubmed:articleTitle |
Reiterated WG/GW motifs form functionally and evolutionarily conserved ARGONAUTE-binding platforms in RNAi-related components.
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pubmed:affiliation |
Laboratoire Génome et Développement de Plantes, Centre National de la Recherche Scientifique/Institut de Recherche pour le Développement/Université de Perpignan 5096, 66860 Perpignan Cedex, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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