Linked Life Data

1793799

Source:http://linkedlifedata.com/resource/pubmed/id/1793799

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1992-4-6
pubmed:abstractText
A method is described for quantitative analysis of the pyrimidopurinone adduct (M1G) arising from reaction of malondialdehyde (MDA) with DNA. DNA samples treated with MDA are reduced with sodium borohydride and hydrolyzed with 0.1 N HCl. The bases released are isolated by solid-phase extraction and analyzed by high-performance liquid chromatography with electrochemical detection. 5,6-Dihydro-M1G (H2M1G) is detected with a glassy carbon electrode at an applied potential of 700 mV vs Ag/AgCl. At this potential, interference from normal deoxynucleoside bases is low. The limit of detection of H2M1G eluted from an HPLC column is 100-200 fmol. The method described should be useful for quantitation of M1G in a variety of DNA samples and biological fluids.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0893-228X
pubmed:author
pubmed:issnType
Print
pubmed:volume
4
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
520-4
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:articleTitle
High-performance liquid chromatography with electrochemical detection for determination of the major malondialdehyde-guanine adduct.
pubmed:affiliation
Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't