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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2007-10-31
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pubmed:abstractText |
We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1548-7091
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pubmed:author |
pubmed-author:AlbertThomas JTJ,
pubmed-author:GibbsRichard ARA,
pubmed-author:MiddleChris MCM,
pubmed-author:MollaMichael NMN,
pubmed-author:MuznyDonna MDM,
pubmed-author:NazarethLynneL,
pubmed-author:PackardCharles JCJ,
pubmed-author:RichmondTodd ATA,
pubmed-author:RodeschMatthew JMJ,
pubmed-author:SongXingzhiX,
pubmed-author:WeinstockGeorge MGM,
pubmed-author:WheelerDavidD
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pubmed:issnType |
Print
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pubmed:volume |
4
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
903-5
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pubmed:meshHeading |
pubmed-meshheading:17934467-BRCA1 Protein,
pubmed-meshheading:17934467-Cell Line, Tumor,
pubmed-meshheading:17934467-Gene Library,
pubmed-meshheading:17934467-Genome, Human,
pubmed-meshheading:17934467-Humans,
pubmed-meshheading:17934467-Nucleic Acid Hybridization,
pubmed-meshheading:17934467-Oligodeoxyribonucleotides,
pubmed-meshheading:17934467-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:17934467-Polymerase Chain Reaction,
pubmed-meshheading:17934467-Polymorphism, Single Nucleotide,
pubmed-meshheading:17934467-Sequence Analysis, DNA
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pubmed:year |
2007
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pubmed:articleTitle |
Direct selection of human genomic loci by microarray hybridization.
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pubmed:affiliation |
NimbleGen Systems Inc., 1 Science Court, Madison, Wisconsin 53711, USA. talbert@nimblegen.com
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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