Linked Life Data

17931752

Source:http://linkedlifedata.com/resource/pubmed/id/17931752

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
45
pubmed:dateCreated
2007-10-23
pubmed:abstractText
Intradermal vaccination via gene gun efficiently delivers DNA vaccines into dendritic cells (DCs) of the skin, resulting in the activation and priming of antigen-specific T cells in vivo. We have previously demonstrated that intradermal delivery of DNA vaccines encoding single-chain trimer (SCT) composed of the most immunogenic epitope of human papillomavirus type 16 (HPV-16) E6 protein (aa49-57), beta2-microglobulin, and MHC class I heavy chain (SCT-E6) can bypass antigen processing and lead to stable cell-surface presentation of E6 peptides. We also showed that co-administration of DNA vaccines with DNA encoding anti-apoptotic proteins can prolong the survival of DNA-transduced DCs, resulting in significant enhancement of antigen-specific CD8(+) T cell immune responses. In the current study, we hypothesized that combining the SCT strategy and antiapoptotic strategy may further enhance DNA vaccine potency by augmenting antigen-specific CD8(+) T cell immune responses and antitumor effects in vaccinated mice. Here, we show that C57BL/6 mice vaccinated with SCT-E6 DNA combined with antiapoptotic protein Bcl-xL DNA generated enhanced E6-specific CD8(+) T cell immune responses compared to mice vaccinated with SCT-E6 DNA and a non-functional mutant Bcl-xL (mtBcl-xL) DNA. Furthermore, we show that mice treated with SCT-E6 and Bcl-xL DNA generated enhanced anti-tumor effects against E6-expressing tumor cells (TC-1/Luciferase) compared to mice treated with SCT-E6 and mtBcl-xL DNA.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-10706121, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-10837079, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-11907065, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-12750398, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-12840065, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-12960321, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-14666426, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-15297964, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-15800656, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-16551258, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-16915291, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-17356542, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-17408372, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-8548765, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-8596636, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-8837611, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-9143702, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-9724092, http://linkedlifedata.com/resource/pubmed/commentcorrection/17931752-9743526
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0264-410X
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7824-31
pubmed:dateRevised
2010-8-9
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Intradermal administration of DNA vaccines combining a strategy to bypass antigen processing with a strategy to prolong dendritic cell survival enhances DNA vaccine potency.
pubmed:affiliation
Department of Pathology, Johns Hopkins Medical Institutions, Baltimore, MD, USA.
pubmed:publicationType
Journal Article, Research Support, N.I.H., Extramural