Linked Life Data

17931108

Source:http://linkedlifedata.com/resource/pubmed/id/17931108

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-10-12
pubmed:abstractText
BK polyomavirus (BKV) reactivation is associated with a failure of T cell immunity in kidney transplant patients, and may lead to BKV-associated nephropathy (BKVN) and loss of the allograft. BKV reactivation in hematopoietic stem cell transplant recipients is associated with hemorrhagic cystitis. We have investigated T cell responses to overlapping peptide mixtures corresponding to the whole BKV major T antigen (TAg) and major capsid protein (VP1) in peripheral blood mononuclear cell samples from a cohort of healthy BKV-seropositive subjects. The majority of these individuals possessed populations of both CD8(+) and CD4(+) T cells specific for these BKV antigens. After expansion in culture, the majority of the BKV-specific CD4(+) T cells, in addition to expressing CD40L (CD154), secreted both interferon (IFN)-gamma and tumor necrosis factor (TNF)-alpha, contained both granzyme A and granzyme B, and degranulated/mobilized CD107 in response to antigen-specific stimulation. These T cells thus represent potentially functional BKV-specific cytotoxic CD4(+) T lymphocytes. Secretion of both TNF-alpha and IFN-gamma by CD154(+)CD4(+) T cells on BKV-specific stimulation was associated with higher levels of granzyme B and a higher proportion of degranulating cells compared with CD154(+)CD4(+) T cells producing only IFN-gamma or neither cytokine. These healthy subjects also harbored populations of functional CD8(+) T cells specific for one or more of three newly defined HLA-A 02-restricted cytotoxic T lymphocyte epitopes within the BKV TAg as well as two HLA-A 02-restricted epitopes within the BKV VP1 we have previously described. The BKV-specific CD4(+) T cells characterized in this study may play a part in maintaining persistent memory T cell responses to the virus and thus contribute to the immune control of BKV in healthy individuals.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0882-8245
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
379-88
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17931108-Adult, pubmed-meshheading:17931108-Antigens, Viral, pubmed-meshheading:17931108-BK Virus, pubmed-meshheading:17931108-CD4-Positive T-Lymphocytes, pubmed-meshheading:17931108-CD40 Ligand, pubmed-meshheading:17931108-CD8-Positive T-Lymphocytes, pubmed-meshheading:17931108-Capsid Proteins, pubmed-meshheading:17931108-Cell Degranulation, pubmed-meshheading:17931108-Cells, Cultured, pubmed-meshheading:17931108-Cytotoxicity, Immunologic, pubmed-meshheading:17931108-Epitopes, T-Lymphocyte, pubmed-meshheading:17931108-Granzymes, pubmed-meshheading:17931108-Humans, pubmed-meshheading:17931108-Interferon-gamma, pubmed-meshheading:17931108-Leukocytes, Mononuclear, pubmed-meshheading:17931108-Polyomavirus Infections, pubmed-meshheading:17931108-T-Lymphocyte Subsets, pubmed-meshheading:17931108-Tumor Necrosis Factor-alpha, pubmed-meshheading:17931108-Tumor Virus Infections
pubmed:year
2007
pubmed:articleTitle
Functional characterization of BK virus-specific CD4+ T cells with cytotoxic potential in seropositive adults.
pubmed:affiliation
Laboratory of Vaccine Research, Beckman Research Institute of the City of Hope, Duarte, CA 91010, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural