Linked Life Data

17929579

Source:http://linkedlifedata.com/resource/pubmed/id/17929579

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2007-10-12
pubmed:abstractText
A total of 38 endophytic fungus strains were isolated from Taxus chinensis var. mairei by aseptic technique. Genomic DNA was extracted from isolated endophytic fungi and subjected to polymerase chain reaction (PCR) analysis for the presence of Taxus taxadiene synthase (TS) gene, a rate-limiting enzyme gene in the taxol biosynthetic pathway. Twelve out of 38 isolated endophytic fungus strains showed PCR positive for the ts gene. Subsequently, taxol and its related compounds were extracted from culture filtrates and mycelia of the PCR positive strains, separated by column chromatography and analyzed by High Performance Liquid Chromatography and Mass Spectrum. The analysis result showed that 3 strains could produce taxol and its related compounds at the detectible level. This study indicates that molecular detection of the ts gene is an efficient method for primary screening of taxol or its related compounds-producing endophytic fungi which can improve prominently screening efficiency.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0555-1099
pubmed:author
pubmed:issnType
Print
pubmed:volume
43
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
490-4
pubmed:meshHeading
pubmed:articleTitle
Screening of taxol-producing endophytic fungi from Taxus chinensis var. mairei.
pubmed:affiliation
Plant Biotechnology Research Center, School of Agriculture and Biology, Fudan-SJTU- Nottingham Plant Biotechnology R&D Center, Shanghai Jiao Tong University, Shanghai 200030, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't