Source:http://linkedlifedata.com/resource/pubmed/id/17928446
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
41
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pubmed:dateCreated |
2007-10-11
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pubmed:abstractText |
Previous work has demonstrated that the surface glycoprotein (gp120) of human immunodeficiency virus-1 (HIV-1) can induce damage and apoptosis of neurons both in vitro and in vivo. In this report, we provide evidence that double-stranded RNA-activated protein kinase (PKR), a stress kinase, is involved in HIV/gp120-associated neurodegeneration. In cultures of mixed cortical cells, HIV/gp120 increased the protein level of PKR. Additionally, PKR was phosphorylated in neurons but not glia after exposure to gp120. The use of two independent pharmacological inhibitors of PKR activity abrogated neuronal cell death induced by gp120. Cortical neurons from PKR knock-out mice were significantly protected from neurotoxicity induced by gp120, further validating the pivotal proapoptotic function of PKR. gp120-induced phosphorylated PKR localized prominently to neuronal nuclei; PKR inhibition or the NMDA receptor antagonist MK-801 [(+)-5-methyl-10,11-dihydro-5H-dibenzo [a,d] cyclohepten-5,10-imine maleate] abrogated this effect. PKR inactivation also inhibited gp120-induced caspase-3 activation, consistent with its neuroprotective effect. Finally, brain tissue from individuals diagnosed with HIV-associated dementia (HAD), but not HIV infection alone, contained the activated form of PKR, which localized predominantly to neuronal nuclei. Together, these results identify PKR as a critical mediator of gp120 neurotoxicity, suggesting that activation of PKR contributes to the neuronal injury and cell death observed in HAD.
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pubmed:grant |
http://linkedlifedata.com/resource/pubmed/grant/F32NS048767,
http://linkedlifedata.com/resource/pubmed/grant/P01 HD29587,
http://linkedlifedata.com/resource/pubmed/grant/P30 MH062261,
http://linkedlifedata.com/resource/pubmed/grant/R01 AI34039,
http://linkedlifedata.com/resource/pubmed/grant/R01 EY09024,
http://linkedlifedata.com/resource/pubmed/grant/R01 MH073490,
http://linkedlifedata.com/resource/pubmed/grant/R01 NS046994,
http://linkedlifedata.com/resource/pubmed/grant/R01 NS047973,
http://linkedlifedata.com/resource/pubmed/grant/R01 NS050621,
http://linkedlifedata.com/resource/pubmed/grant/R24MH059745,
http://linkedlifedata.com/resource/pubmed/grant/T32AG00252
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1529-2401
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:day |
10
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
11047-55
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:17928446-AIDS Dementia Complex,
pubmed-meshheading:17928446-Adult,
pubmed-meshheading:17928446-Animals,
pubmed-meshheading:17928446-Apoptosis,
pubmed-meshheading:17928446-Cells, Cultured,
pubmed-meshheading:17928446-HIV Envelope Protein gp120,
pubmed-meshheading:17928446-HIV-1,
pubmed-meshheading:17928446-Humans,
pubmed-meshheading:17928446-Mice,
pubmed-meshheading:17928446-Mice, Inbred C57BL,
pubmed-meshheading:17928446-Mice, Knockout,
pubmed-meshheading:17928446-Middle Aged,
pubmed-meshheading:17928446-Neurons,
pubmed-meshheading:17928446-Rats,
pubmed-meshheading:17928446-Rats, Sprague-Dawley,
pubmed-meshheading:17928446-Signal Transduction,
pubmed-meshheading:17928446-eIF-2 Kinase
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pubmed:year |
2007
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pubmed:articleTitle |
Human immunodeficiency virus-1/surface glycoprotein 120 induces apoptosis through RNA-activated protein kinase signaling in neurons.
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pubmed:affiliation |
Molecular and Integrative Neurosciences Department, The Scripps Research Institute, La Jolla, California 92037, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, N.I.H., Extramural,
Validation Studies
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