Linked Life Data

17928210

Source:http://linkedlifedata.com/resource/pubmed/id/17928210

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-9-15
pubmed:abstractText
Twenty-nine staphylococcal isolates from different clinical samples were tested for slime production: phenotypic characterization was carried out using Christensen test (tube test) and Congo red agar plate test (CRA plate test), while the presence and expression of icaA and icaD genes were evaluated by real-time PCR. In 79.3% of studied strains there was a concordance between slime production and presence of icaA and icaD genes, and between lack of slime production and absence of both or only one of the tested genes. In four of five strains where positive phenotype was not associated with the presence of ica genes, gene co-expression (evaluated by mRNA determination) was lacking, while in only a case out of five, there was the presence of transcripts without phenotype. Our study, for the first time, introduces real-time PCR for the detection of both icaA and icaD genes and their mRNA and, furthermore, addresses the relationship between slime phenotype absence and mRNA expression, in clinical isolates of coagulase-negative staphylococci.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1618-0623
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
164
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
522-8
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Phenotypic and genotypic evaluation of slime production by conventional and molecular microbiological techniques.
pubmed:affiliation
Department of Medical Sciences, Institute of Microbiology, University Magna Graecia, Catanzaro, Italy.
pubmed:publicationType
Journal Article, Evaluation Studies