Linked Life Data

17928176

Source:http://linkedlifedata.com/resource/pubmed/id/17928176

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2008-2-1
pubmed:abstractText
We report on the synthesis of a novel gene carrier that has low interaction with serum components, as well as low cytotoxicity. Cationic copolymers composing branched poly(ethylenimine) (PEI) grafted with hydrophilic poly(ethylene glycol) (PEG) and poly(l-lactic acid) (PLLA) or small-molecule oleoyl were synthesized and evaluated as novel gene carriers in this study. The copolymers were complexed with plasmid DNA and the resulting polyplexes were approximately 140nm in diameter and had a positive surface potential (zeta=+13.8mV) at the N/P ratio of 10/1. The experiments showed that copolymers with the oleoyl moiety were superior to the other two copolymers (with PLLA), in terms of in vitro gene transfection efficiency. Safety studies using MTT assay indicated much lower cytotoxicity of the oleoyl polyplexes than the pDNA/PEI complexes. The intracellular behavior of the polyplexes was monitored by confocal laser scanning microscopy, and it was found that the polyplexes were internalized into HeLa cells very effectively. At the same time, the plasmid DNA carried by the oleoyl-containing copolymers was found to localize in the nucleus of the recipient cells. One experiment comparing serum-free and serum-containing media indicated that the oleoyl polyplexes may be able to evade the reticulo-endothelial system (RES) better than the PEI-pDNA complex.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0378-5173
pubmed:author
pubmed:issnType
Print
pubmed:day
28
pubmed:volume
350
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
344-50
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Design, synthesis and in vitro evaluation of a novel "stealth" polymeric gene vector.
pubmed:affiliation
School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't