Linked Life Data

17928056

Source:http://linkedlifedata.com/resource/pubmed/id/17928056

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2007-12-31
pubmed:abstractText
M-Ficolin is a member of the ficolin family of proteins, which is expressed by monocytes. We have determined the expression of this gene in various populations of this lineage in man and found lower levels of M-Ficolin mRNA in the more mature CD14(+)CD16(+) monocytes as compared to the classical CD14(++) monocytes. Monocyte-derived macrophages generated by in vitro culture for 5 days strongly reduced M-Ficolin mRNA and protein. Mature tissue macrophages from the lung and from breast milk also showed a very low level of M-Ficolin transcripts. When cells of the monocytic cell line Mono Mac 6 cell were treated with TLR2 and TLR4 ligands for 24 h then there was an average of 6- and 9-fold induction of the M-Ficolin mRNA, respectively. After 72 h induction was in average 30- and 80-fold for TLR2 and TLR4 stimulation, respectively. Treatment of monocyte-derived macrophages for 3 days with TLR4 ligand gave an average 4-fold induction and alveolar macrophages treated with TLR4 ligand showed a 12-fold induction. These data show that M-Ficolin expression is silenced in macrophages but can be re-activated after prolonged activation via TLRs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0161-5890
pubmed:author
pubmed:issnType
Print
pubmed:volume
45
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1424-30
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Expression of M-Ficolin in human monocytes and macrophages.
pubmed:affiliation
Clinical Cooperation Group, Inflammatory Lung Diseases, GSF-National Research Center for Environment and Health and Asklepios Fachkliniken-Gauting, Robert-Koch Allee 29, D-82131, Gauting/Munich, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't