Linked Life Data

17924080

Source:http://linkedlifedata.com/resource/pubmed/id/17924080

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2007-10-9
pubmed:abstractText
This review addresses whole cell separation and isolation using aqueous two-phase systems based on biocompatible polymers. The physicochemical factors that influence phase separation and systems properties are analysed. Especially, emphasis is given to the polyethylene glycol (PEG) and dextran two-phase systems and to stimuli-responsive soluble-insoluble polymers. The major factors that affect cell partitioning, such as polymer molecular weight and concentration, temperature, ionic species and pH, and affinity extraction, are also evaluated taking into account the cell types and cell surface properties. The applications of aqueous two-phase separation in cell processing are described, namely the new developments in continuous cell partitioning in microdevices and extractive bioconversions with relevance to the biomedical sector.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0724-6145
pubmed:author
pubmed:issnType
Print
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
151-71
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Cell partitioning in aqueous two-phase polymer systems.
pubmed:affiliation
Institute for Biotechnology and Bioengineering, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal. joaquim.cabral@ist.utl.pt
pubmed:publicationType
Journal Article, Review