Linked Life Data

17924079

Source:http://linkedlifedata.com/resource/pubmed/id/17924079

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2007-10-9
pubmed:abstractText
The developing fields of cell and tissue engineering will require cost-effective technologies for delivery of cells to patients. Hollow-fibre affinity cell separation is a monoclonal antibody-based cell separation process whereby monoclonal antibody (ligand) is immobilised onto a stationary substrate, the luminal surface of a parallel array of hollow fibres. Deposited cells are fractionated on the basis of adhesion strength using hollow fibre geometry that generates a well-defined shear stress for cell recovery. In this chapter we present the biophysical basis for the process of ligand-mediated cell adhesion and relate this to the performance of affinity cell separation. We also discuss the hydrodynamics of hollow fibre arrays and the various approaches for modifying polymer substrates with protein ligands. One of the major limiting factors for large-scale epitope selective cell separation will be the prohibitive cost of these affinity processes. Hollow fibre systems offer the promise of providing flexibility and scalability for many of these applications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0724-6145
pubmed:author
pubmed:issnType
Print
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
129-50
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Hollow-fibre affinity cell separation.
pubmed:affiliation
Graduate School of Biomedical Engineering, University of New South Wales, 2052 Sydney, Australia. r.nordon@unsw.edu.au
pubmed:publicationType
Journal Article, Review