|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
4
|
|
pubmed:dateCreated |
1992-4-1
|
|
pubmed:abstractText |
A rapid and specific assay for toxic shock syndrome toxin-1 gene (tst gene) detection in Staphylococcus aureus was developed using the polymerase chain reaction. A two-primer set and an oligonucleotide detection probe were synthesized. After 40 cycles of amplification, detection of a 160-bp amplified DNA fragment was carried out by agarose gel electrophoresis and Southern blot hybridization. This assay was sensitive since it was able to detect 1-10 bacteria. It was also specific since no amplification was documented with DNAs from enterotoxigenic S. aureus or Gram-negative bacteria devoid of the tst gene.
|
|
pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Aug
|
|
pubmed:issn |
0890-8508
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
5
|
|
pubmed:geneSymbol |
tst
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
281-4
|
|
pubmed:dateRevised |
2006-11-15
|
|
pubmed:meshHeading |
pubmed-meshheading:1791849-Bacterial Toxins,
pubmed-meshheading:1791849-DNA Probes,
pubmed-meshheading:1791849-Enterotoxins,
pubmed-meshheading:1791849-Genes, Bacterial,
pubmed-meshheading:1791849-Humans,
pubmed-meshheading:1791849-Polymerase Chain Reaction,
pubmed-meshheading:1791849-Sensitivity and Specificity,
pubmed-meshheading:1791849-Shock, Septic,
pubmed-meshheading:1791849-Staphylococcal Infections,
pubmed-meshheading:1791849-Staphylococcus aureus,
pubmed-meshheading:1791849-Superantigens
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Specific detection of the toxic shock syndrome toxin-1 gene using the polymerase chain reaction.
|
|
pubmed:affiliation |
Institut de Bactériologie, Faculté de Médecine, France.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|
