| pubmed-article:17917278 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17917278 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:17917278 | lifeskim:mentions | umls-concept:C0021853 | lld:lifeskim |
| pubmed-article:17917278 | lifeskim:mentions | umls-concept:C0282560 | lld:lifeskim |
| pubmed-article:17917278 | lifeskim:mentions | umls-concept:C0178539 | lld:lifeskim |
| pubmed-article:17917278 | lifeskim:mentions | umls-concept:C0006931 | lld:lifeskim |
| pubmed-article:17917278 | lifeskim:mentions | umls-concept:C1280500 | lld:lifeskim |
| pubmed-article:17917278 | lifeskim:mentions | umls-concept:C0031164 | lld:lifeskim |
| pubmed-article:17917278 | lifeskim:mentions | umls-concept:C0872351 | lld:lifeskim |
| pubmed-article:17917278 | lifeskim:mentions | umls-concept:C1883709 | lld:lifeskim |
| pubmed-article:17917278 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:17917278 | pubmed:dateCreated | 2007-10-5 | lld:pubmed |
| pubmed-article:17917278 | pubmed:abstractText | Recent studies suggest that capsaicin (Cap), a major constituent of hot pepper, may affect the function and permeability of the intestinal mucosa in vitro. However, the relationships between the dose of Cap and the barrier and/or transporter functions on intestinal epithelial cells are unknown. The aim of this study was to investigate whether Cap initiates cellular injury and alter epithelial permeability in Caco-2 cells. Cellular toxicity, as measured using a lactate dehydrogenase release assay, was not observed at high concentrations of Cap (up to 300 microM). When cell viability was measured by a WST-1 assay (tetrazolium salt-based assay), damage to Caco-2 monolayers was observed at doses of 200 and 300 microM of Cap. The barrier function of tight junctions was assessed by measuring transepithelial electrical resistance (TEER) in Caco-2 cells. Treatment of Caco-2 cells with Cap at doses above 100 microM significantly decreased the TEER compared to treatment with buffer alone for 2 h (p<0.05). We next examined the effects of Cap on the activity of P-glycoprotein (P-gp) found on transcellular transporters. At doses of 100 and 200 microM, Cap inhibited the transport of rhodamine 123 by P-gp-mediated efflux in Caco-2 cells. Cap thus exhibited inhibitory effects on P-gp. The results of this study indicate that Cap, a dietary phytochemical, causes functional and structural changes in Caco-2 cell monolayers at noncytotoxic doses (less than 100 microM of Cap). The concomitant administration of Cap with drugs that are substrates of P-gp might increase the plasma concentrations of such drugs. | lld:pubmed |
| pubmed-article:17917278 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17917278 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17917278 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17917278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17917278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17917278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17917278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17917278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17917278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17917278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17917278 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17917278 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17917278 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:17917278 | pubmed:issn | 0918-6158 | lld:pubmed |
| pubmed-article:17917278 | pubmed:author | pubmed-author:IkedaKenjiK | lld:pubmed |
| pubmed-article:17917278 | pubmed:author | pubmed-author:TanakaKazuhik... | lld:pubmed |
| pubmed-article:17917278 | pubmed:author | pubmed-author:AmanoFumioF | lld:pubmed |
| pubmed-article:17917278 | pubmed:author | pubmed-author:HirotaniYoshi... | lld:pubmed |
| pubmed-article:17917278 | pubmed:author | pubmed-author:KatoRyujiR | lld:pubmed |
| pubmed-article:17917278 | pubmed:author | pubmed-author:IjiriYoshioY | lld:pubmed |
| pubmed-article:17917278 | pubmed:author | pubmed-author:TsukuraYuriY | lld:pubmed |
| pubmed-article:17917278 | pubmed:author | pubmed-author:MoriMayaM | lld:pubmed |
| pubmed-article:17917278 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17917278 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:17917278 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17917278 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17917278 | pubmed:pagination | 1982-6 | lld:pubmed |
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| pubmed-article:17917278 | pubmed:meshHeading | pubmed-meshheading:17917278... | lld:pubmed |
| pubmed-article:17917278 | pubmed:year | 2007 | lld:pubmed |
| pubmed-article:17917278 | pubmed:articleTitle | Effects of capsaicin on cellular damage and monolayer permeability in human intestinal Caco-2 cells. | lld:pubmed |
| pubmed-article:17917278 | pubmed:affiliation | Laboratory of Clinical Pharmacy & Clinical Pharmacokinetics, Osaka University of Pharmaceutical Sciences, Takatsuki, Japan. | lld:pubmed |
| pubmed-article:17917278 | pubmed:publicationType | Journal Article | lld:pubmed |
