Source:http://linkedlifedata.com/resource/pubmed/id/17917278
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
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| pubmed:dateCreated |
2007-10-5
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| pubmed:abstractText |
Recent studies suggest that capsaicin (Cap), a major constituent of hot pepper, may affect the function and permeability of the intestinal mucosa in vitro. However, the relationships between the dose of Cap and the barrier and/or transporter functions on intestinal epithelial cells are unknown. The aim of this study was to investigate whether Cap initiates cellular injury and alter epithelial permeability in Caco-2 cells. Cellular toxicity, as measured using a lactate dehydrogenase release assay, was not observed at high concentrations of Cap (up to 300 microM). When cell viability was measured by a WST-1 assay (tetrazolium salt-based assay), damage to Caco-2 monolayers was observed at doses of 200 and 300 microM of Cap. The barrier function of tight junctions was assessed by measuring transepithelial electrical resistance (TEER) in Caco-2 cells. Treatment of Caco-2 cells with Cap at doses above 100 microM significantly decreased the TEER compared to treatment with buffer alone for 2 h (p<0.05). We next examined the effects of Cap on the activity of P-glycoprotein (P-gp) found on transcellular transporters. At doses of 100 and 200 microM, Cap inhibited the transport of rhodamine 123 by P-gp-mediated efflux in Caco-2 cells. Cap thus exhibited inhibitory effects on P-gp. The results of this study indicate that Cap, a dietary phytochemical, causes functional and structural changes in Caco-2 cell monolayers at noncytotoxic doses (less than 100 microM of Cap). The concomitant administration of Cap with drugs that are substrates of P-gp might increase the plasma concentrations of such drugs.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-(4-iodophenyl)-3-(4-nitrophenyl)-5...,
http://linkedlifedata.com/resource/pubmed/chemical/Capsaicin,
http://linkedlifedata.com/resource/pubmed/chemical/Coloring Agents,
http://linkedlifedata.com/resource/pubmed/chemical/L-Lactate Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/P-Glycoprotein,
http://linkedlifedata.com/resource/pubmed/chemical/Pharmaceutical Preparations,
http://linkedlifedata.com/resource/pubmed/chemical/Rhodamine 123,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts
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| pubmed:status |
MEDLINE
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| pubmed:month |
Oct
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| pubmed:issn |
0918-6158
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
30
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1982-6
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| pubmed:meshHeading |
pubmed-meshheading:17917278-Caco-2 Cells,
pubmed-meshheading:17917278-Capsaicin,
pubmed-meshheading:17917278-Cell Membrane,
pubmed-meshheading:17917278-Cell Membrane Permeability,
pubmed-meshheading:17917278-Cell Survival,
pubmed-meshheading:17917278-Coloring Agents,
pubmed-meshheading:17917278-Data Interpretation, Statistical,
pubmed-meshheading:17917278-Electric Impedance,
pubmed-meshheading:17917278-Enterocytes,
pubmed-meshheading:17917278-Humans,
pubmed-meshheading:17917278-Intestinal Absorption,
pubmed-meshheading:17917278-Intestinal Mucosa,
pubmed-meshheading:17917278-L-Lactate Dehydrogenase,
pubmed-meshheading:17917278-P-Glycoprotein,
pubmed-meshheading:17917278-Pharmaceutical Preparations,
pubmed-meshheading:17917278-Rhodamine 123,
pubmed-meshheading:17917278-Tetrazolium Salts,
pubmed-meshheading:17917278-Tight Junctions
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| pubmed:year |
2007
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| pubmed:articleTitle |
Effects of capsaicin on cellular damage and monolayer permeability in human intestinal Caco-2 cells.
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| pubmed:affiliation |
Laboratory of Clinical Pharmacy & Clinical Pharmacokinetics, Osaka University of Pharmaceutical Sciences, Takatsuki, Japan.
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| pubmed:publicationType |
Journal Article
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