Linked Life Data

17916499

Source:http://linkedlifedata.com/resource/pubmed/id/17916499

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2007-10-5
pubmed:abstractText
In the mosquito Culex pipiens complex (Diptera: Culicidae), the amplification of carboxylesterase genes is an important mechanism providing resistance to organophosphate insecticides. Various amplified alleles at the Ester locus have been identified over the world. In this study, two newly detected Ester alleles, Ester(B10) and Ester(11) (including associated Ester(A11) and Ester(B11)), coding for esterases B10 and A11-B11, respectively, are characterized qualitatively and quantitatively. A high molecular identity is observed both at the nucleotide level and at the deduced amino acid level among the known Ester alleles. Real-time quantitative PCR results suggest 2.5-fold amplification of the Ester(B10) allele, 36.5-fold amplification of the Ester(A11) allele, and 19.1-fold amplification of the Ester(B11) allele. The ca. 2-fold difference in amplification level between Ester(A11) and Ester(B11) may indicate a new model for the esterase amplification. Bioassays show that these two resistant Ester alleles only can confer moderate or low resistance to the tested organophosphate insecticides.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0965-1748
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1131-7
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Characterization of novel esterases in insecticide-resistant mosquitoes.
pubmed:affiliation
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't