Linked Life Data

1791168

Source:http://linkedlifedata.com/resource/pubmed/id/1791168

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1992-4-1
pubmed:abstractText
This work attempted to introduce pre-labelled intact amelogenins into developing rat enamel in vitro. The intention was ultimately to trace the fate of such proteins in the developing enamel matrix and to examine the effect of these and other molecules on ameloblast behavior. The penetration of 125I-amelogenin (25 KDa) directly into the young enamel of rat incisors, in vitro, keeping the enamel organ intact is described. The enamel (an extension approximately 8 mm from the apical end of the tooth) together with the enamel organ, was separated from the underlying dentine and placed over a strip of filter paper covering a well of micro-culture slide filled with Eagle's medium containing 125I labelled amelogenin and incubated at 37 degrees C. The enamel faced the strip of filter paper and so was adjacent to the medium. After 1 h pieces were either washed in cold medium, fixed and embedded in Epoxy-resin, or incubated in cold medium for another 1 to 10 h at 37 degrees C before embedding. One-micron thick sections were processed for autoradiography and the results showed a decreasing gradient of silver grain concentration from the dentino-enamel junction towards the enamel organ. It is expected that by determining the nature of the labelled material incorporated into the enamel and enamel organ the fate of amelogenins could be better understood.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
D
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0301-3952
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
305-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
In vitro penetration of 125I-amelogenin into the enamel and enamel organ of rat incisors.
pubmed:affiliation
Department of Morphology, School of Dentistry of Piracicaba, University of Campinas (FOP/Unicamp), Piracicaba-Sp- Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't