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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
7
pubmed:dateCreated
2008-4-8
pubmed:abstractText
The influence of zinc (Zn) on physiological and biochemical parameters was studied to elucidate the mechanism of Zn resistance in Phragmites australis. Zn concentrations in roots, stems and leaves increased with exogenous Zn concentration, while Zn content in roots was much higher than in shoots. X-ray microanalysis was used to reveal compartments in which Zn accumulated in root cortex. Zinc concentrations followed a gradient with the sequence: intercellular space>cell wall >vacuole >cytoplasm, indicating that most Zn was immobilized in the apoplast or sequestered into the vacuolar lumen. Sequential extraction of various Zn chelates revealed that the ratio of Zn extracted with different extraction media was markedly different. Ethanol, HAc (acetic acid) and NaCl-extractable Zn were dominant in both roots and leaves of P. australis. Zn-binding protein fractions were found in the roots and leaves after gel filtration chromatography, among which a polypeptide with an apparent molecular mass of 14 kDa bound Zn most effectively. Two newly synthesized polypeptides of 58 and 45 kDa appeared under Zn pollution, whereas a prominent fraction of 72 kDa disappeared. The involvement of Zn distribution in plant tissues, subcellular compartments and chelates and Zn-inducing proteins in the acclimation mechanism of P. australis to Zn pollution is discussed.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1618-1328
pubmed:author
pubmed:issnType
Electronic
pubmed:day
5
pubmed:volume
165
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
697-704
pubmed:dateRevised
2009-5-5
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Zinc distribution and zinc-binding forms in Phragmites australis under zinc pollution.
pubmed:affiliation
Department of Biological Science and Technology, Dalian University of Technology, Dalian 116024, PR China. jiangxingyu2002@yahoo.com
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't