Linked Life Data

17910490

Source:http://linkedlifedata.com/resource/pubmed/id/17910490

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2007-10-16
pubmed:abstractText
Many chemical and biological processes are dependent on molecular gradients. We describe a new microfluidic approach that can be used to produce spatiotemporal gradients across two-dimensional surfaces and three-dimensional gels under flow-free conditions. Free diffusion between dynamically replenished flow channels acting as a sink and source is utilized to give rise to stable steady-state gradient profiles. The gradient profile is dictated by the engineered design of the device's gradient-generating region. Different designs can yield both linear and non-linear gradients of varying profiles. More complex gradients can be made by juxtaposing different designs within a single gradient-generating region. By fabricating an array of designs along the gradient-generating region, different gradient profiles can be generated simultaneously, allowing for parallel analysis. Additionally, simple methods of localizing gels into microdevices are demonstrated. The device was characterized by experimentally obtained gradient profiles of fluorescent molecules that corroborated closely with a simulated finite element model.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0743-7463
pubmed:author
pubmed:issnType
Print
pubmed:day
23
pubmed:volume
23
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
10910-2
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Generation of stable complex gradients across two-dimensional surfaces and three-dimensional gels.
pubmed:affiliation
Department of Biomedical Engineering, University of California, Irvine, 3109 Natural Sciences 2, Irvine, California 92697-2715, USA.
pubmed:publicationType
Journal Article