Linked Life Data

17906150

Source:http://linkedlifedata.com/resource/pubmed/id/17906150

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 10
pubmed:dateCreated
2007-10-1
pubmed:abstractText
Three putative alkaline phosphatase genes, phoA, phoC and phoD, were identified in the genome of Streptomyces coelicolor by homology with the amino acid sequence obtained from the PhoA protein of Streptomyces griseus. PhoA and PhoC correspond to broad-spectrum alkaline phosphatases whereas PhoD is similar to a Ca(2+)-dependent phospholipase D of Streptomyces chromofuscus. The phoA and phoD genes were efficiently expressed in R5 medium under phosphate-limited conditions, as shown by studies using the xylE reporter gene, whereas phoC was poorly transcribed under the same conditions. Expression of phoA was clearly PhoP-dependent since it was not transcribed in the S. coelicolor DeltaphoP mutant and was strongly activated under low phosphate concentrations. Similarly, expression of phoD was PhoP-dependent and highly sensitive to phosphate availability. By contrast, expression of phoC was not PhoP-dependent. Electrophoretic mobility shift assays showed that PhoP binds to the phoA and phoD promoters, but not to that of phoC. Footprinting studies with GST-PhoP revealed the presence of a PHO box (two direct 11 nt repeats) in the phoA promoter and two PHO boxes in the promoter of phoD. The transcription start points of the three promoters were identified by primer extension. The transcription start point of phoD coincides with the G of its translation start codon, indicating that this gene is transcribed as a leaderless mRNA. The deduced -10 and -35 regions of phoD (but not those of phoA) overlapped with the PHO boxes in this promoter, suggesting that an excess of PhoP interferes with binding of the RNA polymerase to this promoter. In summary, the three promoters showed clear differences in the modulation of their expression by PhoP.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
1350-0872
pubmed:author
pubmed:issnType
Print
pubmed:volume
153
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3527-37
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:17906150-Alkaline Phosphatase, pubmed-meshheading:17906150-Artificial Gene Fusion, pubmed-meshheading:17906150-Bacterial Proteins, pubmed-meshheading:17906150-Base Sequence, pubmed-meshheading:17906150-Binding Sites, pubmed-meshheading:17906150-Catechol 2,3-Dioxygenase, pubmed-meshheading:17906150-DNA, Bacterial, pubmed-meshheading:17906150-DNA Footprinting, pubmed-meshheading:17906150-Electrophoretic Mobility Shift Assay, pubmed-meshheading:17906150-Escherichia coli Proteins, pubmed-meshheading:17906150-Gene Expression Regulation, Bacterial, pubmed-meshheading:17906150-Genes, Reporter, pubmed-meshheading:17906150-Molecular Sequence Data, pubmed-meshheading:17906150-Phosphates, pubmed-meshheading:17906150-Phospholipase D, pubmed-meshheading:17906150-Promoter Regions, Genetic, pubmed-meshheading:17906150-Protein Binding, pubmed-meshheading:17906150-Streptomyces coelicolor, pubmed-meshheading:17906150-Transcription Initiation Site
pubmed:year
2007
pubmed:articleTitle
Phosphate control of phoA, phoC and phoD gene expression in Streptomyces coelicolor reveals significant differences in binding of PhoP to their promoter regions.
pubmed:affiliation
Instituto de Biotecnología de León, INBIOTEC, Parque Científico de León, Av. Real 1, 24006 León, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't