Linked Life Data

17905400

Source:http://linkedlifedata.com/resource/pubmed/id/17905400

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2007-11-20
pubmed:abstractText
In transgenic zebrafish (Danio rerio), green fluorescent protein (GFP) is a promising marker for environmental pollutants. In using GFP, one of the obstacles which we faced was how to compare toxicity among different toxicants or among a specific toxicant in different model species with the intensity of GFP expression. Using a fluorescence detection method, we first validated our method for estimating the amount of GFP fluorescence present in transgenic fish, which we used as an indicator of developmental toxicity caused by the well-known toxicant, arsenite. To this end, we developed mosaic transgenic zebrafish with the human heat shock response element (HSE) fused to the enhanced GFP (EGFP) reporter gene to indicate exposure to arsenite. We confirmed that EGFP expression sites correlate with gross morphological disruption caused by arsenite exposure. Arsenite (300.0 microM) caused stronger EGFP fluorescence intensity and quantity than 50.0 microM and 10.0 microM arsenite in our transgenic zebrafish. Furthermore, arsenite-induced apoptosis was demonstrated by TUNEL assay. Apoptosis was inhibited by the antioxidant, N-acetyl-cystein (NAC) in this transgenic zebrafish. The distribution of TUNEL-positive cells in embryonic tissues was correlated with the sites of arsenite toxicity and EGFP expression. The EGFP values quantified using the standard curve equation from the known GFP quantity were consistent with the arsenite-induced EGFP expression pattern and arsenite concentration, indicating that this technique can be a reliable and applicable measurement. In conclusion, we propose that fluorescence-based EGFP quantification in transgenic fish containing the hsp70 promoter-EGFP reporter-gene construct is a useful indicator of development toxicity caused by arsenite.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0041-008X
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
225
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
154-61
pubmed:meshHeading
pubmed-meshheading:17905400-Acetylcysteine, pubmed-meshheading:17905400-Animals, pubmed-meshheading:17905400-Animals, Genetically Modified, pubmed-meshheading:17905400-Antioxidants, pubmed-meshheading:17905400-Apoptosis, pubmed-meshheading:17905400-Arsenites, pubmed-meshheading:17905400-Biological Markers, pubmed-meshheading:17905400-Dose-Response Relationship, Drug, pubmed-meshheading:17905400-Embryo, Nonmammalian, pubmed-meshheading:17905400-Environmental Pollutants, pubmed-meshheading:17905400-Fluorescent Dyes, pubmed-meshheading:17905400-Gene Expression Regulation, Developmental, pubmed-meshheading:17905400-Genes, Reporter, pubmed-meshheading:17905400-Green Fluorescent Proteins, pubmed-meshheading:17905400-HSP70 Heat-Shock Proteins, pubmed-meshheading:17905400-In Situ Nick-End Labeling, pubmed-meshheading:17905400-Mosaicism, pubmed-meshheading:17905400-Zebrafish
pubmed:year
2007
pubmed:articleTitle
Quantitative GFP fluorescence as an indicator of arsenite developmental toxicity in mosaic heat shock protein 70 transgenic zebrafish.
pubmed:affiliation
Department of Laboratory Animal Medicine, College of Veterinary Medicine and KRF Zoonotic Disease Priority Research Institute, Seoul National University, Seoul 151-742, Republic of Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't