Source:http://linkedlifedata.com/resource/pubmed/id/17896076
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2008-1-21
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pubmed:abstractText |
The aim of this study was to analyze molecular features of protease-resistant prion protein (PrP(res)) in Western blots of BSE cases diagnosed in Poland with respect to a possible atypical status. Confirmed cases were analyzed by Western blotting with several monoclonal antibodies directed at N-terminal and core epitopes of prion protein (PrP). Most cases showed the classical glycoprofile characterized by the dominance of the di- over the monoglycosylated PrP(res) band, yielding di-/mono- ratios well above 2 and by reactivity with antibodies having their epitopes in bovine PrP region 110-242 (C-type cases). Surprisingly, seven cases of BSE were atypical. Six were classified as L-type based on a slightly lower molecular mass (M(r)) of the non- glycosylated band with respect to C-types and a conspicuously low di-/mono- ratio of glycosylated PrP(res) bands approaching unity. One case was classified as H-type because of a higher M(r) of PrP(res) bands on the blot when compared with C-type cases. A characteristic epitope of H-type PrP(res) occurred in the 101-110 region of PrP for which only antibody 12B2 had a sufficient affinity. The occurrence of atypical cases only in animals 9 years of age and older raises questions about the mechanisms of prion diseases and the origin of BSE.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0304-8608
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
153
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
69-79
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pubmed:meshHeading |
pubmed-meshheading:17896076-Animals,
pubmed-meshheading:17896076-Blotting, Western,
pubmed-meshheading:17896076-Brain,
pubmed-meshheading:17896076-Cattle,
pubmed-meshheading:17896076-Encephalopathy, Bovine Spongiform,
pubmed-meshheading:17896076-Glycosylation,
pubmed-meshheading:17896076-Poland,
pubmed-meshheading:17896076-PrPC Proteins,
pubmed-meshheading:17896076-PrPSc Proteins,
pubmed-meshheading:17896076-Prion Diseases,
pubmed-meshheading:17896076-Prions,
pubmed-meshheading:17896076-Retrospective Studies,
pubmed-meshheading:17896076-Scrapie
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pubmed:year |
2008
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pubmed:articleTitle |
Atypical status of bovine spongiform encephalopathy in Poland: a molecular typing study.
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pubmed:affiliation |
National Veterinary Research Institute, Pulawy, Poland. ppolak@piwet.pulawy.pl
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pubmed:publicationType |
Journal Article
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