Linked Life Data

17893858

Source:http://linkedlifedata.com/resource/pubmed/id/17893858

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2007-10-22
pubmed:abstractText
Epidemiological and laboratory evidence indicate that, in addition to tobacco and alcohol, human papillomaviruses (HPV) play an important aetiological role in a subset of head and neck squamous cell carcinoma (HNSCC). To evaluate the molecular pathogenesis of HPV-infected HNSCC, we compared gene expression patterns between HPV-positive and -negative HNSCC tumours using cDNA microarrays. Tumour tissue was collected from 42 histologically confirmed HNSCC patients from an inner-city area of New York. Total DNA and RNA were extracted and purified from frozen tumour samples and gene expression levels were compared to a universal human reference RNA standard using a 27 323 cDNA microarray chip. HPV detection and genotyping were performed using an MY09/11-PCR system and RT-PCR. HPV was detected in 29% of HNSCC tumours. Most harboured only HPV16 and expressed the HPV16-E6 oncogene. HPV prevalence was highest in pharyngeal tumours (45%). Gene expression patterns that differentiated HPV-positive from negative tumours were compared by supervised classification analysis, and a multiple-gene signature was found to predict HPV16 prevalence in primary HNSCC with a false discovery rate < 0.2. Focusing on never-smokers, we further identified a distinct subset of 123 genes that were specifically dysregulated in HPV16-positive HNSCC. Overexpressed genes in HPV-positive HNSCC tumours included the retinoblastoma-binding protein (p18), replication factor-C gene, and an E2F-dimerization partner transcription factor (TFDP2) that have also been found to be overexpressed in cervical cancer. An additional subset of genes involved in viral defence and immune response, including interleukins and interferon-induced proteins, was found to be down-regulated in HPV-positive tumours, supporting a characteristic and unique transcriptional profile in HPV-induced HNSCC.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-3417
pubmed:author
pubmed:copyrightInfo
Copyright 2007 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
pubmed:issnType
Print
pubmed:volume
213
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
283-93
pubmed:meshHeading
pubmed-meshheading:17893858-Aged, pubmed-meshheading:17893858-Alcohol Drinking, pubmed-meshheading:17893858-Carcinoma, Squamous Cell, pubmed-meshheading:17893858-DNA, Viral, pubmed-meshheading:17893858-Female, pubmed-meshheading:17893858-Gene Expression Profiling, pubmed-meshheading:17893858-Head and Neck Neoplasms, pubmed-meshheading:17893858-Human papillomavirus 16, pubmed-meshheading:17893858-Humans, pubmed-meshheading:17893858-Linear Models, pubmed-meshheading:17893858-Male, pubmed-meshheading:17893858-Middle Aged, pubmed-meshheading:17893858-Oligonucleotide Array Sequence Analysis, pubmed-meshheading:17893858-Oncogene Proteins, Viral, pubmed-meshheading:17893858-Oncogenes, pubmed-meshheading:17893858-Papillomavirus Infections, pubmed-meshheading:17893858-RNA, Viral, pubmed-meshheading:17893858-Repressor Proteins, pubmed-meshheading:17893858-Smoking
pubmed:year
2007
pubmed:articleTitle
Gene expression profiles in HPV-infected head and neck cancer.
pubmed:affiliation
Department of Epidemiology & Population Health, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA. nschlech@aecom.yu.edu
pubmed:publicationType
Journal Article, Comparative Study, Research Support, N.I.H., Extramural