Linked Life Data

17893810

Source:http://linkedlifedata.com/resource/pubmed/id/17893810

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2007-9-25
pubmed:abstractText
Raman spectroscopy is emerging as a powerful method for obtaining both quantitative and qualitative information from biological samples. One very interesting area of research, for which the technique has rarely been used, is the detection, quantification and structural analysis of post-translational modifications (PTMs) on proteins. Since Raman spectra can be used to address both of these questions simultaneously, we have developed near infrared Raman spectroscopy with appropriate chemometric approaches (partial least squares regression) to quantify low concentration (4 microM) mixtures of phosphorylated and dephosphorylated bovine alpha(s)-casein. In addition, we have used these data in conjunction with Raman optical activity (ROA) spectra and NMR to assess the structural changes that occur upon phosphorylation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0003-2654
pubmed:author
pubmed:issnType
Print
pubmed:volume
132
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1053-60
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Quantification of casein phosphorylation with conformational interpretation using Raman spectroscopy.
pubmed:affiliation
Manchester Interdisciplinary Biocentre, 131 Princess Street, Manchester, UKM1 7ND. roger.jarvis@manchester.ac.uk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't