| pubmed-article:17889820 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:17889820 | lifeskim:mentions | umls-concept:C1521828 | lld:lifeskim |
| pubmed-article:17889820 | lifeskim:mentions | umls-concept:C0597731 | lld:lifeskim |
| pubmed-article:17889820 | lifeskim:mentions | umls-concept:C1510941 | lld:lifeskim |
| pubmed-article:17889820 | lifeskim:mentions | umls-concept:C1948059 | lld:lifeskim |
| pubmed-article:17889820 | lifeskim:mentions | umls-concept:C1720529 | lld:lifeskim |
| pubmed-article:17889820 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:17889820 | pubmed:dateCreated | 2007-12-19 | lld:pubmed |
| pubmed-article:17889820 | pubmed:abstractText | Surface plasmon resonance imaging systems, such as Flexchip from Biacore, are capable of monitoring hundreds of reaction spots simultaneously within a single flow cell. Interpreting the binding kinetics in a large-format flow cell presents a number of potential challenges, including accounting for mass transport effects and spot-to-spot sample depletion. We employed a combination of computer simulations and experimentation to characterize these effects across the spotted array and established that a simple two-compartment model may be used to accurately extract intrinsic rate constants from the array under mass transport-limited conditions. Using antibody systems, we demonstrate that the spot-to-spot variability in the binding kinetics was <9%. We also illustrate the advantage of globally fitting binding data from multiple spots within an array for a system that is mass transport limited. | lld:pubmed |
| pubmed-article:17889820 | pubmed:language | eng | lld:pubmed |
| pubmed-article:17889820 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17889820 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:17889820 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:17889820 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:17889820 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:17889820 | pubmed:issn | 0003-2697 | lld:pubmed |
| pubmed-article:17889820 | pubmed:author | pubmed-author:MyszkaDavid... | lld:pubmed |
| pubmed-article:17889820 | pubmed:author | pubmed-author:RichRebecca... | lld:pubmed |
| pubmed-article:17889820 | pubmed:author | pubmed-author:CannonMichell... | lld:pubmed |
| pubmed-article:17889820 | pubmed:author | pubmed-author:LambertJeremy... | lld:pubmed |
| pubmed-article:17889820 | pubmed:author | pubmed-author:SundaramShank... | lld:pubmed |
| pubmed-article:17889820 | pubmed:author | pubmed-author:JenkinsJerryJ | lld:pubmed |
| pubmed-article:17889820 | pubmed:author | pubmed-author:PandianPrabha... | lld:pubmed |
| pubmed-article:17889820 | pubmed:author | pubmed-author:MagyarRachell... | lld:pubmed |
| pubmed-article:17889820 | pubmed:author | pubmed-author:BrockmanJenni... | lld:pubmed |
| pubmed-article:17889820 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:17889820 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:17889820 | pubmed:volume | 373 | lld:pubmed |
| pubmed-article:17889820 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:17889820 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:17889820 | pubmed:pagination | 112-20 | lld:pubmed |
| pubmed-article:17889820 | pubmed:meshHeading | pubmed-meshheading:17889820... | lld:pubmed |
| pubmed-article:17889820 | pubmed:meshHeading | pubmed-meshheading:17889820... | lld:pubmed |
| pubmed-article:17889820 | pubmed:meshHeading | pubmed-meshheading:17889820... | lld:pubmed |
| pubmed-article:17889820 | pubmed:meshHeading | pubmed-meshheading:17889820... | lld:pubmed |
| pubmed-article:17889820 | pubmed:meshHeading | pubmed-meshheading:17889820... | lld:pubmed |
| pubmed-article:17889820 | pubmed:meshHeading | pubmed-meshheading:17889820... | lld:pubmed |
| pubmed-article:17889820 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:17889820 | pubmed:articleTitle | Extracting kinetic rate constants from surface plasmon resonance array systems. | lld:pubmed |
| pubmed-article:17889820 | pubmed:affiliation | Center for Biomolecular Interaction Analysis, School of Medicine, University of Utah, Salt Lake City, UT 84132, USA. | lld:pubmed |
| pubmed-article:17889820 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:17889820 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17889820 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:17889820 | lld:pubmed |
