| pubmed-article:178822 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:178822 | lifeskim:mentions | umls-concept:C0000742 | lld:lifeskim |
| pubmed-article:178822 | lifeskim:mentions | umls-concept:C0005955 | lld:lifeskim |
| pubmed-article:178822 | lifeskim:mentions | umls-concept:C1510411 | lld:lifeskim |
| pubmed-article:178822 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:178822 | lifeskim:mentions | umls-concept:C0392762 | lld:lifeskim |
| pubmed-article:178822 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:178822 | pubmed:dateCreated | 1976-8-2 | lld:pubmed |
| pubmed-article:178822 | pubmed:abstractText | A quantitative Abelson murine leukemia virus (A-MuLV) lymphoid cell transformation assay has been developed using a semisolid agarose culture system. Under these conditions lymphoid cell transformation was shown to vary linearly with the dose of A-MuLV used. The susceptibility of bone marrow cells from different strains of mice to A-MuLV-induced transformation can be estimated using the agarose assay. Strains with bone marrow cells of high, medium, and low susceptibility to A-MuLV can be identified. The assay has been used to study the susceptibility of cells from lymphoid organs of fetal and adult mice to A-MuLV. Cell suspensions from fetal liver, adult bone marrow, and adult spleen are susceptible to A-MuLV, while thymocytes are resistant to A-MuLV-induced transformation. Bovine serum albumin gradient fractionation of bone marrow cells before infection with A-MuLV demonstrates that the majority of A-MuLV-sensitive cells are recovered in a broad band partially overlapping the majority of the nucleated cells. The agarose assay system allows study of A-MuLV-lymphoid cell interaction at the level of single cell-single virus particle interaction. | lld:pubmed |
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| pubmed-article:178822 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:178822 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:178822 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:178822 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:178822 | pubmed:issn | 0022-1007 | lld:pubmed |
| pubmed-article:178822 | pubmed:author | pubmed-author:BaltimoreDD | lld:pubmed |
| pubmed-article:178822 | pubmed:author | pubmed-author:RosenbergNN | lld:pubmed |
| pubmed-article:178822 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:178822 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:178822 | pubmed:volume | 143 | lld:pubmed |
| pubmed-article:178822 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:178822 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:178822 | pubmed:pagination | 1453-63 | lld:pubmed |
| pubmed-article:178822 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:178822 | pubmed:year | 1976 | lld:pubmed |
| pubmed-article:178822 | pubmed:articleTitle | A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus. | lld:pubmed |
| pubmed-article:178822 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:178822 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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