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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1976-8-2
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pubmed:abstractText |
A quantitative Abelson murine leukemia virus (A-MuLV) lymphoid cell transformation assay has been developed using a semisolid agarose culture system. Under these conditions lymphoid cell transformation was shown to vary linearly with the dose of A-MuLV used. The susceptibility of bone marrow cells from different strains of mice to A-MuLV-induced transformation can be estimated using the agarose assay. Strains with bone marrow cells of high, medium, and low susceptibility to A-MuLV can be identified. The assay has been used to study the susceptibility of cells from lymphoid organs of fetal and adult mice to A-MuLV. Cell suspensions from fetal liver, adult bone marrow, and adult spleen are susceptible to A-MuLV, while thymocytes are resistant to A-MuLV-induced transformation. Bovine serum albumin gradient fractionation of bone marrow cells before infection with A-MuLV demonstrates that the majority of A-MuLV-sensitive cells are recovered in a broad band partially overlapping the majority of the nucleated cells. The agarose assay system allows study of A-MuLV-lymphoid cell interaction at the level of single cell-single virus particle interaction.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-1079572,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-1103381,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-1127379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-14187925,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-163444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-163975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-165315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-168584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-171081,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-4099080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-4112916,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-4141645,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-4175476,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-4308055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-4318922,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-4347027,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-4355680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-4372605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-4608884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/178822-4986654
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
143
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1453-63
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:178822-Animals,
pubmed-meshheading:178822-Bone Marrow,
pubmed-meshheading:178822-Bone Marrow Cells,
pubmed-meshheading:178822-Cell Transformation, Neoplastic,
pubmed-meshheading:178822-Clone Cells,
pubmed-meshheading:178822-Leukemia Virus, Murine,
pubmed-meshheading:178822-Lymphoid Tissue,
pubmed-meshheading:178822-Mice,
pubmed-meshheading:178822-Mice, Inbred Strains,
pubmed-meshheading:178822-Receptors, Antigen, B-Cell,
pubmed-meshheading:178822-Species Specificity
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pubmed:year |
1976
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pubmed:articleTitle |
A quantitative assay for transformation of bone marrow cells by Abelson murine leukemia virus.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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