Source:http://linkedlifedata.com/resource/pubmed/id/17880362
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
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| pubmed:dateCreated |
2007-9-20
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| pubmed:abstractText |
1. It has been well established that oestrogens can increase the number of endothelial progenitor cells (EPC) by anti-apoptotic effects. Resveratrol, a polyphenolic phytoalexin extracted from grapes and wine, has been reported to act as an oestrogen receptor agonist. We hypothesize that putative phyto-oestrogen may promote EPC proliferation and survival in vitro. 2. Endothelial progenitor cells were isolated from human peripheral blood and identified immunocytochemically. Endothelial progenitor cells were incubated with resveratrol (1, 10, 25 and 50 mmol/L) or control for specified times. Cell proliferation, migration and in vitro vasculogenesis were assayed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetra-zolium bromide (MTT) assay, modified Boyden chamber assay and in vitro vasculogenesis detection, respectively. 3. Resveratrol increased the number of EPC and promoted EPC proliferation, adhesion and migration in a dose- and time-dependent manner. Cell number peaked at 50 mmol/L resveratrol after incubation for 24 h compared with vehicle control (61.3 +/- 5.8 vs 112.8 +/- 7.2, respectively; P < 0.01). 4. Furthermore, cell cycle analysis showed that 50 mmol/L resveratrol significantly increased the S phase and decreased the G(0)/G(1) phase of EPC. In addition, resveratrol increased vascular endothelial growth factor production and further induced vasculogenesis in vitro. 5. In conclusion, resveratrol significantly induces EPC proliferation, migration and further promotes angiogenesis in vitro.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Phytoestrogens,
http://linkedlifedata.com/resource/pubmed/chemical/Stilbenes,
http://linkedlifedata.com/resource/pubmed/chemical/VEGFA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A,
http://linkedlifedata.com/resource/pubmed/chemical/resveratrol
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0305-1870
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
34
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1109-15
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| pubmed:meshHeading |
pubmed-meshheading:17880362-Cell Adhesion,
pubmed-meshheading:17880362-Cell Differentiation,
pubmed-meshheading:17880362-Cell Movement,
pubmed-meshheading:17880362-Cell Proliferation,
pubmed-meshheading:17880362-Cell Survival,
pubmed-meshheading:17880362-Cells, Cultured,
pubmed-meshheading:17880362-Dose-Response Relationship, Drug,
pubmed-meshheading:17880362-Endothelial Cells,
pubmed-meshheading:17880362-G0 Phase,
pubmed-meshheading:17880362-G1 Phase,
pubmed-meshheading:17880362-Humans,
pubmed-meshheading:17880362-Neovascularization, Physiologic,
pubmed-meshheading:17880362-Phytoestrogens,
pubmed-meshheading:17880362-S Phase,
pubmed-meshheading:17880362-Stem Cells,
pubmed-meshheading:17880362-Stilbenes,
pubmed-meshheading:17880362-Time Factors,
pubmed-meshheading:17880362-Vascular Endothelial Growth Factor A
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| pubmed:year |
2007
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| pubmed:articleTitle |
Effects of resveratrol on number and activity of endothelial progenitor cells from human peripheral blood.
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| pubmed:affiliation |
Department of Cardiology, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu, China. wangxiaobin@medmail.com.cn
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| pubmed:publicationType |
Journal Article
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