17878710

Source:http://linkedlifedata.com/resource/pubmed/id/17878710

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Predicate	Object
rdf:type	pubmed:Citation
lifeskim:mentions	umls-concept:C0012854, umls-concept:C0018270, umls-concept:C0025663, umls-concept:C0031809, umls-concept:C0039717, umls-concept:C0456962, umls-concept:C0598689, umls-concept:C1527148
pubmed:issue	4
pubmed:dateCreated	2008-2-27
pubmed:abstractText	There is a lack of relevant methods to assess the colonization of textiles by skin bacteria because present methods are mainly culture-based procedures. Therefore, the goal of this study was to develop a fast and sensitive culture-independent procedure for the quantification of microbial colonization and growth on textiles. We have established a suitable protocol to use DNA quantification as a reliable method for in vitroand in vivoinvestigations of textiles. For DNA extraction, a two-step procedure comprising treatment of the textile with a solution containing Triton X-100 and lysozyme for 1 h and a successive treatment by SDS and proteinase K for 2 h turned out to be most efficient. DNA extracted from textiles and fabrics was than quantified with the highly sensitive PicoGreen fluorescent dye. In vitrochallenge tests demonstrated a strong correlation between numbers of bacteria on textiles and amount of DNA extracted from textiles. Therefore, this method was used to compare different materials after in vivotrials for assessment of their susceptibility for microbial colonization and growth.
pubmed:language	eng
pubmed:journal	http://linkedlifedata.com/resource/pubmed/journal/100892561
pubmed:citationSubset	IM
pubmed:chemical	http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial
pubmed:status	MEDLINE
pubmed:issn	1660-2412
pubmed:author	pubmed-author:BechtoldThomasT, pubmed-author:MerschakPetraP, pubmed-author:RedlBernhardB, pubmed-author:SchusterK ChristianKC, pubmed-author:TeufelLindaL
pubmed:copyrightInfo	(c) 2007 S. Karger AG, Basel
pubmed:issnType	Electronic
pubmed:volume	14
pubmed:owner	NLM
pubmed:authorsComplete	Y
pubmed:pagination	193-200
pubmed:meshHeading	pubmed-meshheading:17878710-Bacteria, pubmed-meshheading:17878710-Bacteriological Techniques, pubmed-meshheading:17878710-DNA, Bacterial, pubmed-meshheading:17878710-Reproducibility of Results, pubmed-meshheading:17878710-Textiles
pubmed:year	2008
pubmed:articleTitle	Development of a fast and reliable method for the assessment of microbial colonization and growth on textiles by DNA quantification.
pubmed:affiliation	Christian Doppler Laboratory for Textile and Fibre Chemistry in Cellulosics, Institute of Textile Chemistry and Textile Physics, Leopold-Franzens University Innsbruck, Dornbirn, Austria.
pubmed:publicationType	Journal Article, Research Support, Non-U.S. Gov't