Linked Life Data

17876098

Source:http://linkedlifedata.com/resource/pubmed/id/17876098

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2007-9-18
pubmed:abstractText
The movement of immune cells is an indispensable prerequisite for their function. All essential steps of cellular immunity rely on the ability of cells to migrate and to interact with each other. Although observation of these phenomena in vivo would be the most physiological approach, intravital imaging is technically very demanding and not optimally suited for routine or high-throughput analysis. Any good in vitro experimental system should reflect the inherent three-dimensionality of cell migration and interaction in living tissues. Data generated over the last decade show that important cellular parameters like cell velocity, cell shape, and the physicodynamics of cell-cell interactions closely resemble values observed in vivo when measured in a three-dimensional (3D) collagen matrix assay, featuring a hydrated network of fibers consisting of type I collagen, the major component of the extracellular matrix. In this chapter, we describe in detail the experimental use of the 3D collagen matrix system. We delineate the preparation of immune cells exemplified by bone marrow-derived dendritic cells and antigen specific T-helper cells of the mouse, the build-up and use of the 3D collagen matrix chamber, the procedures of real time fluorescence microscopic analysis of cell migration and cell-cell interaction, as well as data analysis supported by a self-developed software for computer-assisted cell tracking.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
380
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
253-69
pubmed:meshHeading
pubmed:year
2007
pubmed:articleTitle
Analyzing the physicodynamics of immune cells in a three-dimensional collagen matrix.
pubmed:affiliation
Helmholtz Centre for Infection Research, Braunschweig, Germany.
pubmed:publicationType
Journal Article, Review