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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1992-3-25
|
| pubmed:abstractText |
We observed a wide distribution of the carbohydrate epitopes galactosyl alpha(1-3)galactose (gal alpha1-3 gal), alpha-glucoside and alpha-mannoside in mono- and heteroxenic trypanosomatids by using fluorescein-labelled lectins of Euonymus europaeus (EE) and Concanavalin A (Con A) as well as sera from acute chagasic patients who have very high levels of anti-gal alpha(1-3)gal antibodies. The direct fluorescence test for gal alpha1-3 gal with EE was positive at minimum concentrations of 6 micrograms/ml for heteroxenic trypanosomatids and 0.7 micrograms/ml for monoxenic ones and for the plant parasite, Phytomonas. On the other hand, heteroxenic trypanosomatids that infect vertebrates bound ten-fold more Con A than monoxenic flagellates and Phytomonas. These data were confirmed in ELISA and Western Blot assays carried out with peroxidase-labelled EE and Con A. Euonymus europaeus recognized several glycoproteins in all trypanosomatids that we tested. Con A, however, recognized a glycoprotein cluster in heteroxenic protozoa, which ranging from 60-120 kDa, seemed to lack monoxenic parasites and Phytomonas. These findings suggest that alpha-D-mannose and alpha-D-glucose might play an important role in the interaction between trypanosomatids and vertebrate hosts.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carbohydrates,
http://linkedlifedata.com/resource/pubmed/chemical/Concanavalin A,
http://linkedlifedata.com/resource/pubmed/chemical/Galactose,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0022-3921
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
38
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
320-5
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:1787421-Alleles,
pubmed-meshheading:1787421-Animals,
pubmed-meshheading:1787421-Anura,
pubmed-meshheading:1787421-Blotting, Western,
pubmed-meshheading:1787421-Carbohydrate Metabolism,
pubmed-meshheading:1787421-Carbohydrate Sequence,
pubmed-meshheading:1787421-Carbohydrates,
pubmed-meshheading:1787421-Chagas Disease,
pubmed-meshheading:1787421-Chiroptera,
pubmed-meshheading:1787421-Concanavalin A,
pubmed-meshheading:1787421-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1787421-Fluorescent Antibody Technique,
pubmed-meshheading:1787421-Galactose,
pubmed-meshheading:1787421-Glycoproteins,
pubmed-meshheading:1787421-Horses,
pubmed-meshheading:1787421-Humans,
pubmed-meshheading:1787421-Lectins,
pubmed-meshheading:1787421-Molecular Sequence Data,
pubmed-meshheading:1787421-Plant Lectins,
pubmed-meshheading:1787421-Plants,
pubmed-meshheading:1787421-Protozoan Proteins,
pubmed-meshheading:1787421-Rodentia,
pubmed-meshheading:1787421-Trypanosoma
|
| pubmed:articleTitle |
Distribution of carbohydrates recognized by the lectins Euonymus europaeus and concanavalin A in monoxenic and heteroxenic trypanosomatids.
|
| pubmed:affiliation |
Centro de Pesquisas René Rachou, Belo Horizonte, MG, Brazil.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|