Source:http://linkedlifedata.com/resource/pubmed/id/17873368
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2007-9-17
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pubmed:abstractText |
Gabapentin (neurontin), a GABA analogue anticonvulsant has proven to be effective in anti-nociceptive activity as well as for the treatment of anxiety. Gabapentin (GBP) is well tolerated and shows very favorable side effects profile: The exact molecular mechanism of action of GBP to block postoperative pain and stress is not known. Therefore, to identify the functional neuroanatomical target sites of GBP in post-surgery as well as its effect on postsurgical process, we examined the effects of GBP on c-Fos expression in the supraspinal part of the central nervous system in rats. Using a well-validated rat model of surgical pain, we studied the neuroanatomical functional target sites of gabapentin after paw surgery. The effect of GBP was examined by means of c-Fos immunohistochemistry. A single intraperitoneal injection (i.p.) of GBP (150 mg/kg) or saline (control) was administered 20 min before surgical incision in the paw under anesthesia. Ninety minutes after surgical incision, the deeply anesthetized rats were perfused transcardially with 4% paraformaldehyde. Serial 40-microm-thick sections of whole brain (except spinal cord) were cut and processed by immunohistochemistry to locate and quantify the sites and number of neurons with c-Fos immunoreactivity. Detection of c-Fos protein was performed using the peroxidase-antiperoxidase detection protocol. Our present study demonstrated that compared to control, administration of GBP (150 mg/kg, i.p.) before paw surgery significantly (p < 0.01) attenuated the incision-induced c-Fos expression only in the paraventricular nucleus of the hypothalamus. In addition, GBP-induced increase in c-Fos expression was observed in the dorsal raphe (DRN) and in the nucleus raphe magnus. Present results indicate that GBP may differentially modulate c-Fos expression in surgical paw incision. Moreover, this study provides some clue to examine whether GBP exerts its action simultaneously through two separate pathways in post-surgery.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amines,
http://linkedlifedata.com/resource/pubmed/chemical/Analgesics,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclohexanecarboxylic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/gabapentin,
http://linkedlifedata.com/resource/pubmed/chemical/gamma-Aminobutyric Acid
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pubmed:status |
MEDLINE
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pubmed:issn |
0895-8696
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
228-34
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pubmed:meshHeading |
pubmed-meshheading:17873368-Amines,
pubmed-meshheading:17873368-Analgesics,
pubmed-meshheading:17873368-Animals,
pubmed-meshheading:17873368-Brain,
pubmed-meshheading:17873368-Cyclohexanecarboxylic Acids,
pubmed-meshheading:17873368-Gene Expression Regulation,
pubmed-meshheading:17873368-Genes, fos,
pubmed-meshheading:17873368-Hindlimb,
pubmed-meshheading:17873368-Kinetics,
pubmed-meshheading:17873368-Male,
pubmed-meshheading:17873368-Neurons,
pubmed-meshheading:17873368-Pain, Postoperative,
pubmed-meshheading:17873368-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:17873368-Rats,
pubmed-meshheading:17873368-Rats, Sprague-Dawley,
pubmed-meshheading:17873368-gamma-Aminobutyric Acid
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pubmed:year |
2007
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pubmed:articleTitle |
Effect of gabapentin on c-Fos expression in the CNS after paw surgery in rats.
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pubmed:affiliation |
Department of Anaesthesia, Yong Loo Lin School of Medicine, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074, Singapore. anaajk@nus.edu.sg
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pubmed:publicationType |
Journal Article
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