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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2007-9-20
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pubmed:abstractText |
Because platelets are anucleate cells having a limited life span, direct gene manipulation cannot in principle be used to investigate the involvement of a specific signal transduction pathway in platelet activation. In this study, we examined whether the expression of a short hairpin RNA (shRNA) sequence in hematopoietic stem cells is maintained during megakaryocyte differentiation, thus resulting in inhibition of targeted protein in platelets.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1524-4636
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2266-72
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:17872456-Animals,
pubmed-meshheading:17872456-Blood Platelets,
pubmed-meshheading:17872456-Bone Marrow Transplantation,
pubmed-meshheading:17872456-Cell Line, Tumor,
pubmed-meshheading:17872456-Cell Shape,
pubmed-meshheading:17872456-Genes, Reporter,
pubmed-meshheading:17872456-Genetic Vectors,
pubmed-meshheading:17872456-Green Fluorescent Proteins,
pubmed-meshheading:17872456-Hematopoietic Stem Cells,
pubmed-meshheading:17872456-Humans,
pubmed-meshheading:17872456-Lentivirus,
pubmed-meshheading:17872456-Megakaryocytes,
pubmed-meshheading:17872456-Mice,
pubmed-meshheading:17872456-Platelet Adhesiveness,
pubmed-meshheading:17872456-Platelet Glycoprotein GPIIb-IIIa Complex,
pubmed-meshheading:17872456-Platelet Glycoprotein GPIb-IX Complex,
pubmed-meshheading:17872456-Platelet Membrane Glycoprotein IIb,
pubmed-meshheading:17872456-Promoter Regions, Genetic,
pubmed-meshheading:17872456-RNA, Small Interfering,
pubmed-meshheading:17872456-RNA Interference,
pubmed-meshheading:17872456-RNA Polymerase III,
pubmed-meshheading:17872456-Signal Transduction,
pubmed-meshheading:17872456-Talin,
pubmed-meshheading:17872456-Thrombopoiesis,
pubmed-meshheading:17872456-Transduction, Genetic
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pubmed:year |
2007
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pubmed:articleTitle |
Silencing of a targeted protein in in vivo platelets using a lentiviral vector delivering short hairpin RNA sequence.
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pubmed:affiliation |
Research Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University School of Medicine, 3111-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan. tohmori@jichi.ac.jp
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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